1bb8: Difference between revisions

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[[Image:1bb8.gif|left|200px]]
{{Seed}}
[[Image:1bb8.png|left|200px]]


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{{STRUCTURE_1bb8|  PDB=1bb8  |  SCENE=  }}  
{{STRUCTURE_1bb8|  PDB=1bb8  |  SCENE=  }}  


'''N-TERMINAL DNA BINDING DOMAIN FROM TN916 INTEGRASE, NMR, 25 STRUCTURES'''
===N-TERMINAL DNA BINDING DOMAIN FROM TN916 INTEGRASE, NMR, 25 STRUCTURES===




==Overview==
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The integrase family of site-specific recombinases catalyze a diverse array of DNA rearrangements in archaebacteria, eubacteria and yeast. The solution structure of the DNA binding domain of the integrase protein from the conjugative transposon Tn916 has been determined using NMR spectroscopy. The structure provides the first insights into distal site DNA binding by a site-specific integrase and reveals that the N-terminal domain is structurally similar to the double stranded RNA binding domain (dsRBD). The results of chemical shift mapping experiments suggest that the integrase protein interacts with DNA using residues located on the face of its three stranded beta-sheet. This surface differs from the proposed RNA binding surface in dsRBDs, suggesting that different surfaces on the same protein fold can be used to bind DNA and RNA.
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(as it appears on PubMed at http://www.pubmed.gov), where 9665166 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9665166}}


==About this Structure==
==About this Structure==
1BB8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BB8 OCA].  
1BB8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BB8 OCA].  


==Reference==
==Reference==
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[[Category: Integrase]]
[[Category: Integrase]]
[[Category: Transposition]]
[[Category: Transposition]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 11:17:59 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:45:04 2008''

Revision as of 15:45, 30 June 2008

File:1bb8.png

Template:STRUCTURE 1bb8

N-TERMINAL DNA BINDING DOMAIN FROM TN916 INTEGRASE, NMR, 25 STRUCTURES

Template:ABSTRACT PUBMED 9665166

About this Structure

1BB8 is a Single protein structure of sequence from Enterococcus faecalis. Full experimental information is available from OCA.

Reference

Site-specific DNA binding using a variation of the double stranded RNA binding motif., Connolly KM, Wojciak JM, Clubb RT, Nat Struct Biol. 1998 Jul;5(7):546-50. PMID:9665166

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