5gm5

Crystal structure of FI-CMCase from Aspergillus aculeatus F-50 in complex with cellobiose

spinqualitylabelsall models 5gm5, resolution 1.73Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5gm5 is a 7 chain structure with sequence from Aspergillus aculeatus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.73Å Ligands: BGC, EPE, PRD_900005, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function GUN_ASPAC Publication Abstract from PubMed Cellulose is the major component of the plant cell wall and the most abundant renewable biomass on earth, and its decomposition has proven to be very useful in many commercial applications. Endo-1,4-beta-d-glucanase (EC 3.2.1.4; endoglucanase), which catalyzes the random hydrolysis of 1,4-beta-glycosidic bonds of the cellulose main chain to cleave cellulose into smaller fragments, is the key cellulolytic enzyme. An endoglucanase isolated from Aspergillus aculeatus F-50 (FI-CMCase), which is classified into the glycoside hydrolase (GH) family 12, was demonstrated to be effectively expressed in the industrial strain Pichia pastoris. Here, the crystal structure and complex structures of P. pastoris-expressed FI-CMCase were solved to high resolution. The overall structure is analyzed and compared to other GH12 members. In addition, the substrate-surrounding residues were engineered to search for variants with improved enzymatic activity. Among 14 mutants constructed, one with two-fold increase in protein expression was identified, which possesses a potential to be further developed as a commercial enzyme product. Crystal structure and genetic modifications of FI-CMCase from Aspergillus aculeatus F-50.,Huang JW, Liu W, Lai HL, Cheng YS, Zheng Y, Li Q, Sun H, Kuo CJ, Guo RT, Chen CC Biochem Biophys Res Commun. 2016 Sep 16;478(2):565-72. doi:, 10.1016/j.bbrc.2016.07.101. Epub 2016 Jul 25. PMID:27470581 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Glucanase 3D structures

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