1bc4: Difference between revisions

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[[Image:1bc4.jpg|left|200px]]
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[[Image:1bc4.png|left|200px]]


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{{STRUCTURE_1bc4|  PDB=1bc4  |  SCENE=  }}  
{{STRUCTURE_1bc4|  PDB=1bc4  |  SCENE=  }}  


'''THE SOLUTION STRUCTURE OF A CYTOTOXIC RIBONUCLEASE FROM THE OOCYTES OF RANA CATESBEIANA (BULLFROG), NMR, 15 STRUCTURES'''
===THE SOLUTION STRUCTURE OF A CYTOTOXIC RIBONUCLEASE FROM THE OOCYTES OF RANA CATESBEIANA (BULLFROG), NMR, 15 STRUCTURES===




==Overview==
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RC-RNase is a pyrimidine-guanine sequence-specific ribonuclease and a lectin possessing potent cell cytotoxicity. It was isolated from the oocytes of Rana catesbeiana (bull frog). From analysis of an extensive set of 1H homonuclear 2D NMR spectra we have completed the resonance assignments. Determination of the three-dimensional structure was carried out with the program X-PLOR using a total of 951 restraints including 814 NMR-derived distances, 61 torsion angles, and 76 hydrogen bond restraints. In the resultant family of 15 best structures, selected from a total of 150 calculated structures, the root-mean-square deviation from the average structure for the backbone heavy-atoms involved in well-defined secondary structure is 0.48 A, while that for all backbone heavy-atoms is 0.91 A. The structure of RC-RNase consists of three alpha-helices and two triple-stranded anti-parallel beta-sheets and folds in a kidney-shape, very similar to the X-ray crystal structure of a homolo gous protein, onconase isolated from Rana pipiens. We have also investigated the interaction between RC-RNase and two inhibitors, cytidylyl(2'--&gt;5')guanosine (2',5'-CpG) and 2'-deoxycytidylyl(3'--&gt;5')-2'-deoxyguanosine (3',5'-dCpdG). Based on the ligand-induced chemical shift changes in RC-RNase and the NOE cross-peaks between RC-RNase and the inhibitors, the key residues involved in protein-inhibitor interaction have been identified. The inhibitors were found to bind in a "retro-binding" mode, with the guanine base bonded to the B1 subsite. The His103 residue was found to occupy the B state with the imidazole ring pointing away from the active site. The structure coordinates and the NMR restraints have been deposited in the Brookhaven Protein Data Bank (1bc4 and 1bc4mr, respectively).
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{{ABSTRACT_PUBMED_9761686}}


==About this Structure==
==About this Structure==
1BC4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rana_catesbeiana Rana catesbeiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BC4 OCA].  
1BC4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rana_catesbeiana Rana catesbeiana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BC4 OCA].  


==Reference==
==Reference==
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[[Category: Rc-rnase]]
[[Category: Rc-rnase]]
[[Category: Sialic acid binding lectin]]
[[Category: Sialic acid binding lectin]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 11:19:31 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:48:16 2008''

Revision as of 15:48, 30 June 2008

File:1bc4.png

Template:STRUCTURE 1bc4

THE SOLUTION STRUCTURE OF A CYTOTOXIC RIBONUCLEASE FROM THE OOCYTES OF RANA CATESBEIANA (BULLFROG), NMR, 15 STRUCTURES

Template:ABSTRACT PUBMED 9761686

About this Structure

1BC4 is a Single protein structure of sequence from Rana catesbeiana. Full experimental information is available from OCA.

Reference

The solution structure of a cytotoxic ribonuclease from the oocytes of Rana catesbeiana (bullfrog)., Chang CF, Chen C, Chen YC, Hom K, Huang RF, Huang TH, J Mol Biol. 1998;283(1):231-44. PMID:9761686

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