6ww7: Difference between revisions
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==Structure of the human ER membrane protein complex in a lipid nanodisc== | ==Structure of the human ER membrane protein complex in a lipid nanodisc== | ||
<StructureSection load='6ww7' size='340' side='right'caption='[[6ww7]]' scene=''> | <StructureSection load='6ww7' size='340' side='right'caption='[[6ww7]], [[Resolution|resolution]] 3.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WW7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WW7 FirstGlance]. <br> | <table><tr><td colspan='2'>[[6ww7]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WW7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WW7 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ww7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ww7 OCA], [http://pdbe.org/6ww7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ww7 RCSB], [http://www.ebi.ac.uk/pdbsum/6ww7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ww7 ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ww7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ww7 OCA], [http://pdbe.org/6ww7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ww7 RCSB], [http://www.ebi.ac.uk/pdbsum/6ww7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ww7 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Disease == | |||
[[http://www.uniprot.org/uniprot/EMC1_HUMAN EMC1_HUMAN]] Global developmental delay-visual anomalies-progressive cerebellar atrophy-truncal hypotonia syndrome. The disease is caused by mutations affecting the gene represented in this entry. | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/EMC10_HUMAN EMC10_HUMAN]] Promotes angiogenesis and tissue repair in the heart after myocardial infarction. Stimulates cardiac endothelial cell migration and outgrowth via the activation of p38 MAPK, PAK and MAPK2 signaling pathways.<ref>PMID:28931551</ref> [[http://www.uniprot.org/uniprot/MMGT1_HUMAN MMGT1_HUMAN]] Mediates Mg(2+) transport.[UniProtKB:Q8K273] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A defining step in the biogenesis of a membrane protein is the insertion of its hydrophobic transmembrane helices into the lipid bilayer. The nine-subunit ER membrane protein complex (EMC) is a conserved co- and post-translational insertase at the endoplasmic reticulum. We determined the structure of the human EMC in a lipid nanodisc to an overall resolution of 3.4 A by cryo-electron microscopy, permitting building of a nearly complete atomic model. We used structure-guided mutagenesis to demonstrate that substrate insertion requires a methionine-rich cytosolic loop and occurs via an enclosed hydrophilic vestibule within the membrane formed by the subunits EMC3 and EMC6. We propose that the EMC uses local membrane thinning and a positively charged patch to decrease the energetic barrier for insertion into the bilayer. | |||
Structural basis for membrane insertion by the human ER membrane protein complex.,Pleiner T, Pinton Tomaleri G, Januszyk K, Inglis AJ, Hazu M, Voorhees RM Science. 2020 May 21. pii: science.abb5008. doi: 10.1126/science.abb5008. PMID:32439656<ref>PMID:32439656</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6ww7" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Inglis | [[Category: Inglis, A J]] | ||
[[Category: Januszyk K]] | [[Category: Januszyk, K]] | ||
[[Category: Pleiner T]] | [[Category: Pleiner, T]] | ||
[[Category: Tomaleri | [[Category: Tomaleri, G P]] | ||
[[Category: Voorhees | [[Category: Voorhees, R M]] | ||
[[Category: Endoplasmic reticulum]] | |||
[[Category: Insertase]] | |||
[[Category: Membrane protein]] | |||
[[Category: Transmembrane chaperone]] | |||
Revision as of 06:42, 3 June 2020
Structure of the human ER membrane protein complex in a lipid nanodisc
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