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| {{STRUCTURE_1bf6| PDB=1bf6 | SCENE= }} | | {{STRUCTURE_1bf6| PDB=1bf6 | SCENE= }} |
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| '''PHOSPHOTRIESTERASE HOMOLOGY PROTEIN FROM ESCHERICHIA COLI'''
| | ===PHOSPHOTRIESTERASE HOMOLOGY PROTEIN FROM ESCHERICHIA COLI=== |
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| ==Overview==
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| Phosphotriesterase homology protein (PHP) is a member of a recently discovered family of proteins related to phosphotriesterase, a hydrolytic, bacterial enzyme with an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates, which are common constituents of chemical warfare agents and agricultural pesticides. No natural substrate has been identified for phosphotriesterase, and it has been suggested that the enzyme may have evolved the ability to hydrolyze synthetic compounds in bacteria under selective pressure to meet nutritional needs. PHP, which has 28% sequence identity with phosphotriesterase, may belong to the family of proteins from which phosphotriesterase evolved. Here we report the cloning, expression, initial characterization, and high-resolution X-ray crystallographic structure of PHP. Biochemical analysis shows that PHP is monomeric and binds two zinc ions per monomer. Unlike phosphotriesterase, PHP does not catalyze the hydrolysis of nonspecific phosphotriesters. The structure, similar to that of phosphotriesterase, consists of a long, elliptical alpha/beta barrel and has a binuclear zinc center in a cleft at the carboxy end of the barrel at the location of the presumptive active site.
| | The line below this paragraph, {{ABSTRACT_PUBMED_9548740}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 9548740 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_9548740}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Hypothetical protein]] | | [[Category: Hypothetical protein]] |
| [[Category: Phosphotriesterase]] | | [[Category: Phosphotriesterase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:26:11 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:01:26 2008'' |
