5ic9: Difference between revisions

Revision as of 13:45, 30 August 2023

Structure of the CTD complex of Utp12 and Utp13

Structural highlights

5ic9 is a 4 chain structure with sequence from Chaetomium thermophilum var. thermophilum DSM 1495. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.7Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

G0RZL9_CHATD

Publication Abstract from PubMed

Ribosome assembly is an essential and conserved cellular process in eukaryotes that requires numerous assembly factors. The six-subunit UTPB complex is an essential component of the 90S precursor of the small ribosomal subunit. Here, we analyzed the molecular architecture of UTPB using an integrative structural biology approach. We mapped the major interactions that associate each of six UTPB proteins. Crystallographic studies showed that Utp1, Utp21, Utp12 and Utp13 are evolutionarily related and form a dimer of dimers (Utp1-Utp21, Utp12-Utp13) through their homologous helical C-terminal domains. Molecular docking with crosslinking restraints showed that the WD domains of Utp12 and Utp13 are associated, as are the WD domains of Utp1, Utp21 and Utp18. Electron microscopy images of the entire UTPB complex revealed that it predominantly adopts elongated conformations and possesses internal flexibility. We also determined crystal structures of the WD domain of Utp18 and the HAT and deviant HAT domains of Utp6. A structural model of UTPB was derived based on these data.

Integrative structural analysis of the UTPB complex, an early assembly factor for eukaryotic small ribosomal subunits.,Zhang C, Sun Q, Chen R, Chen X, Lin J, Ye K Nucleic Acids Res. 2016 Jun 21. pii: gkw562. PMID:27330138^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhang C, Sun Q, Chen R, Chen X, Lin J, Ye K. Integrative structural analysis of the UTPB complex, an early assembly factor for eukaryotic small ribosomal subunits. Nucleic Acids Res. 2016 Jun 21. pii: gkw562. PMID:27330138 doi:https://dx.doi.org/10.1093/nar/gkw562

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OCA

[1] Zhang C, Sun Q, Chen R, Chen X, Lin J, Ye K. Integrative structural analysis of the UTPB complex, an early assembly factor for eukaryotic small ribosomal subunits. Nucleic Acids Res. 2016 Jun 21. pii: gkw562. PMID:27330138 doi:https://dx.doi.org/10.1093/nar/gkw562

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='5ic9' size='340' side='right'caption='[[5ic9]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ic9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IC9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5IC9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ic9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IC9 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5ic7|5ic7]], [[5ic8|5ic8]], [[5ica|5ica]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.7&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CTHT_0003430 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD]), CTHT_0065530 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ic9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ic9 OCA], [https://pdbe.org/5ic9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ic9 RCSB], [https://www.ebi.ac.uk/pdbsum/5ic9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ic9 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ic9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ic9 OCA], [http://pdbe.org/5ic9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ic9 RCSB], [http://www.ebi.ac.uk/pdbsum/5ic9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ic9 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/G0RZL9_CHATD G0RZL9_CHATD]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Chatd]]
+[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]]
 [[Category: Large Structures]]
-[[Category: Ye, K]]
+[[Category: Ye K]]
-[[Category: Zhang, C]]
+[[Category: Zhang C]]
-[[Category: 90s preribosome]]
-[[Category: Protein binding]]
-[[Category: Rrna processing]]
-[[Category: Solenoid structure]]
-[[Category: Structural protein]]

5ic9: Difference between revisions

Revision as of 13:45, 30 August 2023

Structure of the CTD complex of Utp12 and Utp13

Structural highlights

Function

Publication Abstract from PubMed

References

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