6v6h: Difference between revisions
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==Crystal structure of histidine ammonia-lyase from Trypanosoma cruzi== | ==Crystal structure of histidine ammonia-lyase from Trypanosoma cruzi== | ||
<StructureSection load='6v6h' size='340' side='right'caption='[[6v6h]]' scene=''> | <StructureSection load='6v6h' size='340' side='right'caption='[[6v6h]], [[Resolution|resolution]] 2.55Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V6H OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[6v6h]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V6H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6V6H FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene>, <scene name='pdbligand=MDO:{2-[(1S)-1-AMINOETHYL]-4-METHYLIDENE-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>MDO</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6v6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v6h OCA], [https://pdbe.org/6v6h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6v6h RCSB], [https://www.ebi.ac.uk/pdbsum/6v6h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6v6h ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q4E133_TRYCC Q4E133_TRYCC] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Chagas disease is one of seventeen neglected tropical diseases according to the World Health Organization (WHO). The histidine-glutamate metabolic pathway is an oxidative route that has shown to be relevant for the bioenergetics in Trypanosoma cruzi, the etiological agent for Chagas disease. Histidine ammonia-lyase participates in the first stage of the histidine catabolism, catalyzing the conversion of l-histidine into urocanate. This work presents the three-dimensional (3D) structure of Trypanosoma cruzi histidine ammonia-lyase enzyme (TcHAL) and some comparisons of it to homologous structures. The enzyme was expressed, purified and assayed for crystallization, what allowed the obtainment of crystals of sufficient quality to collect X-ray diffraction data up to 2.55A resolution. After refinement, some structural analyses indicated that the structure does not contain the active site protection domain, in opposition to previously known 3D structures from plants and fungi phenylalanine ammonia-lyase, therefore, it is the first structure of eukaryotic ammonia-lyases that lacks this domain. | |||
Crystal structure of histidine ammonia-lyase from Trypanosoma cruzi.,Miranda RR, Silva M, Barison MJ, Silber AM, Iulek J Biochimie. 2020 May 25. pii: S0300-9084(20)30118-8. doi:, 10.1016/j.biochi.2020.05.009. PMID:32464165<ref>PMID:32464165</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6v6h" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Trypanosoma cruzi]] | |||
[[Category: Barison MJ]] | [[Category: Barison MJ]] | ||
[[Category: Iulek J]] | [[Category: Iulek J]] | ||
Revision as of 08:04, 11 October 2023
Crystal structure of histidine ammonia-lyase from Trypanosoma cruzi
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