6k8p

Structural and catalytic analysis of two diverse uridine phosphorylases in the oomycete Phytophthora capsici.

spinqualitylabelsall models 6k8p, resolution 1.96Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6k8p is a 4 chain structure with sequence from Phytophthora capsici LT1534. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.965Å Ligands: PO4, R1P, TDR, THM Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A410UCT3_PHYCP Publication Abstract from PubMed Uridine phosphorylase (UP) is a key enzyme of pyrimidine salvage pathways that enables the recycling of endogenous or exogenous-supplied pyrimidines and plays an important intracellular metabolic role. Here, we biochemically and structurally characterized two evolutionarily divergent uridine phosphorylases, PcUP1 and PcUP2 from the oomycete pathogen Phytophthora capsici. Our analysis of other oomycete genomes revealed that both uridine phosphorylases are present in Phytophthora and Pythium genomes, but only UP2 is seen in Saprolegnia spp. which are basal members of the oomycetes. Moreover, uridine phosphorylases are not found in obligate oomycete pathogens such as Hyaloperonospora arabidopsidis and Albugo spp. PcUP1 and PcUP2 are upregulated 300 and 500 fold respectively, within 90 min after infection of pepper leaves. The crystal structures of PcUP1 in ligand-free and in complex with uracil/ribose-1-phosphate, 2'-deoxyuridine/phosphate and thymidine/phosphate were analyzed. Crystal structure of this uridine phosphorylase showed strict conservation of key residues in the binding pocket. Structure analysis of PcUP1 with bound ligands, and site-directed mutagenesis of key residues provide additional support for the "push-pull" model of catalysis. Our study highlights the importance of pyrimidine salvage during the earliest stages of infection. Structural and catalytic analysis of two diverse uridine phosphorylases in Phytophthora capsici.,Yang C, Li J, Huang Z, Zhang X, Gao X, Zhu C, Morris PF, Zhang X Sci Rep. 2020 Jun 3;10(1):9051. doi: 10.1038/s41598-020-65935-9. PMID:32493959 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Uridine phosphorylase 3D structures

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