1etg: Difference between revisions
New page: left|200px<br /> <applet load="1etg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1etg" /> '''REV RESPONSE ELEMENT (RRE) RNA COMPLEXED WI... |
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[[Image:1etg.gif|left|200px]]<br /> | [[Image:1etg.gif|left|200px]]<br /><applet load="1etg" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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'''REV RESPONSE ELEMENT (RRE) RNA COMPLEXED WITH REV PEPTIDE, NMR, 19 STRUCTURES'''<br /> | '''REV RESPONSE ELEMENT (RRE) RNA COMPLEXED WITH REV PEPTIDE, NMR, 19 STRUCTURES'''<br /> | ||
==Overview== | ==Overview== | ||
The solution structure of a human immunodeficiency virus type-1 (HIV-1) | The solution structure of a human immunodeficiency virus type-1 (HIV-1) Rev peptide bound to stem-loop IIB of the Rev response element (RRE) RNA was solved by nuclear magnetic resonance spectroscopy. The Rev peptide has an alpha-helical conformation and binds in the major groove of the RNA near a purine-rich internal loop. Several arginine side chains make base-specific contacts, and an asparagine residue contacts a G.A base pair. The phosphate backbone adjacent to a G.G base pair adopts an unusual structure that allows the peptide to access a widened major groove. The structure formed by the two purine-purine base pairs of the RRE creates a distinctive binding pocket that the peptide can use for specific recognition. | ||
==About this Structure== | ==About this Structure== | ||
1ETG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http:// | 1ETG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETG OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Human immunodeficiency virus 1]] | [[Category: Human immunodeficiency virus 1]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Battiste, J | [[Category: Battiste, J L.]] | ||
[[Category: Frankel, A | [[Category: Frankel, A D.]] | ||
[[Category: Kay, L | [[Category: Kay, L E.]] | ||
[[Category: Mao, H.]] | [[Category: Mao, H.]] | ||
[[Category: Muhandiram, D | [[Category: Muhandiram, D R.]] | ||
[[Category: Rao, N | [[Category: Rao, N S.]] | ||
[[Category: Tan, R.]] | [[Category: Tan, R.]] | ||
[[Category: Willamson, J | [[Category: Willamson, J R.]] | ||
[[Category: complex (rna/protein)]] | [[Category: complex (rna/protein)]] | ||
[[Category: export regulator]] | [[Category: export regulator]] | ||
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[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:31:24 2008'' | ||
Revision as of 10:31, 21 February 2008
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REV RESPONSE ELEMENT (RRE) RNA COMPLEXED WITH REV PEPTIDE, NMR, 19 STRUCTURES
Overview
The solution structure of a human immunodeficiency virus type-1 (HIV-1) Rev peptide bound to stem-loop IIB of the Rev response element (RRE) RNA was solved by nuclear magnetic resonance spectroscopy. The Rev peptide has an alpha-helical conformation and binds in the major groove of the RNA near a purine-rich internal loop. Several arginine side chains make base-specific contacts, and an asparagine residue contacts a G.A base pair. The phosphate backbone adjacent to a G.G base pair adopts an unusual structure that allows the peptide to access a widened major groove. The structure formed by the two purine-purine base pairs of the RRE creates a distinctive binding pocket that the peptide can use for specific recognition.
About this Structure
1ETG is a Single protein structure of sequence from Human immunodeficiency virus 1. Full crystallographic information is available from OCA.
Reference
Alpha helix-RNA major groove recognition in an HIV-1 rev peptide-RRE RNA complex., Battiste JL, Mao H, Rao NS, Tan R, Muhandiram DR, Kay LE, Frankel AD, Williamson JR, Science. 1996 Sep 13;273(5281):1547-51. PMID:8703216
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