1bwo: Difference between revisions

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[[Image:1bwo.jpg|left|200px]]
{{Seed}}
[[Image:1bwo.png|left|200px]]


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{{STRUCTURE_1bwo|  PDB=1bwo  |  SCENE=  }}  
{{STRUCTURE_1bwo|  PDB=1bwo  |  SCENE=  }}  


'''THE CRYSTAL STRUCTURE OF WHEAT NON-SPECIFIC TRANSFER PROTEIN COMPLEXED WITH TWO MOLECULES OF PHOSPHOLIPID AT 2.1 A RESOLUTION'''
===THE CRYSTAL STRUCTURE OF WHEAT NON-SPECIFIC TRANSFER PROTEIN COMPLEXED WITH TWO MOLECULES OF PHOSPHOLIPID AT 2.1 A RESOLUTION===




==Overview==
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Nonspecific lipid transfer proteins (ns-LTP1) form a multigenic protein family in plants. In vitro they are able to bind all sort of lipids but their function, in vivo, remains speculative. A ns-LTP1 isolated from wheat seed was crystallized in the presence of lyso-myristoyl-phosphatidylcholine (LMPC). The structure was solved by molecular replacement and refined to 2.1 A resolution to an R-factor of 16.3% and a free R-factor of 21.3%. It reveals for the first time that the protein binds two LMPC molecules that are inserted head to tail in a hydrophobic cavity. A detailed study of the structure leads to the conclusion that there are two lipid-binding sites, one of which shows a higher affinity for the LMPC than the other. Comparison with other structures of lipid-bound ns-LTP1 suggests that the presence of two binding sites is a general feature of plant ns-LTP1.
The line below this paragraph, {{ABSTRACT_PUBMED_10491104}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_10491104}}


==About this Structure==
==About this Structure==
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[[Category: Lipid transfer protein]]
[[Category: Lipid transfer protein]]
[[Category: Wheat]]
[[Category: Wheat]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 12:02:46 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:50:06 2008''

Revision as of 16:50, 30 June 2008

File:1bwo.png

Template:STRUCTURE 1bwo

THE CRYSTAL STRUCTURE OF WHEAT NON-SPECIFIC TRANSFER PROTEIN COMPLEXED WITH TWO MOLECULES OF PHOSPHOLIPID AT 2.1 A RESOLUTION

Template:ABSTRACT PUBMED 10491104

About this Structure

1BWO is a Single protein structure of sequence from Triticum aestivum. Full crystallographic information is available from OCA.

Reference

The crystal structure of a wheat nonspecific lipid transfer protein (ns-LTP1) complexed with two molecules of phospholipid at 2.1 A resolution., Charvolin D, Douliez JP, Marion D, Cohen-Addad C, Pebay-Peyroula E, Eur J Biochem. 1999 Sep;264(2):562-8. PMID:10491104

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