NudT16: Difference between revisions
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== Function == | == Function == | ||
HsNudT16 has a variety of functions including hydrolysis of certain biomolecules and keeping the cell free of harmful nucleotides. It is responsible for hydrolyzing inosine triphosphate or diphosphate. This protein also has a large substrate specificity and has mRNA decapping capability. | |||
Thirawatananond et. al. investigated whether the widening of the ADPr binding site would allow for increased hydrolysis activity by NudT16. The group designed such mutants (F36A, F61S, and a double mutant with both F36A and F61S) and found that for these mutants, NudT16 hydrolysis activity decreased in free ADPr, remained comparably efficient in mono(ADP-ribosylated) proteins, and increased in poly(ADP-ribosylated) proteins. <ref>PMID: 30976021</ref> | |||
NudT16 is a (Deoxy)inosine diphosphatase. Iyama et. al. found that HsNudT16 binds strongly to GTP, ITP, and XTP. The loss of this protein is followed by increased accumulation of single-strand breaks in DNA, reduced proliferation, and increased cell arrest. They also found increased levels of inosine in RNA, which informed the conclusion that HsNudT16 functions in the nucleus to protect the cell from ITP and its detrimental effects. <ref>PMID:20385596</ref> | |||
