1c0e: Difference between revisions

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[[Image:1c0e.jpg|left|200px]]
{{Seed}}
[[Image:1c0e.png|left|200px]]


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{{STRUCTURE_1c0e|  PDB=1c0e  |  SCENE=  }}  
{{STRUCTURE_1c0e|  PDB=1c0e  |  SCENE=  }}  


'''ACTIVE SITE S19A MUTANT OF BOVINE HEART PHOSPHOTYROSYL PHOSPHATASE'''
===ACTIVE SITE S19A MUTANT OF BOVINE HEART PHOSPHOTYROSYL PHOSPHATASE===




==Overview==
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The bovine protein tyrosine phosphatase (BPTP) is a member of the class of low-molecular weight protein tyrosine phosphatases (PTPases) found to be ubiquitous in mammalian cells. The catalytic site of BPTP contains a CX(5)R(S/T) phosphate-binding motif or P-loop (residues 12-19) which is the signature sequence for all PTPases. Ser19, the final residue of the P-loop motif, interacts with the catalytic Cys12 and participates in stabilizing the conformation of the active site through interactions with Asn15, also in the P-loop. Mutations at Ser19 result in an enzyme with altered kinetic properties with changes in the pK(a) of the neighboring His72. The X-ray structure of the S19A mutant enzyme shows that the general conformation of the P-loop is preserved. However, changes in the loop containing His72 result in a displacement of the His72 side chain that may explain the shift in the pK(a). In addition, it was found that in the crystal, the protein forms a dimer in which Tyr131 and Tyr132 from one monomer insert into the active site of the other monomer, suggesting a dual-tyrosine motif on target sites for this enzyme. Since the activity of this PTPase is reportedly regulated by phosphorylation at Tyr131 and Tyr132, the structure of this dimer may provide a model of a self-regulation mechanism for the low-molecular weight PTPases.
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{{ABSTRACT_PUBMED_10512620}}


==About this Structure==
==About this Structure==
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[[Category: Phosphatase dimer]]
[[Category: Phosphatase dimer]]
[[Category: Tyrosine phosphatase]]
[[Category: Tyrosine phosphatase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 12:11:12 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:02:13 2008''

Revision as of 17:02, 30 June 2008

File:1c0e.png

Template:STRUCTURE 1c0e

ACTIVE SITE S19A MUTANT OF BOVINE HEART PHOSPHOTYROSYL PHOSPHATASE

Template:ABSTRACT PUBMED 10512620

About this Structure

1C0E is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

The structure of the bovine protein tyrosine phosphatase dimer reveals a potential self-regulation mechanism., Tabernero L, Evans BN, Tishmack PA, Van Etten RL, Stauffacher CV, Biochemistry. 1999 Sep 7;38(36):11651-8. PMID:10512620

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