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| [[Image:1c2p.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1c2p| PDB=1c2p | SCENE= }} | | {{STRUCTURE_1c2p| PDB=1c2p | SCENE= }} |
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| '''HEPATITIS C VIRUS NS5B RNA-DEPENDENT RNA POLYMERASE'''
| | ===HEPATITIS C VIRUS NS5B RNA-DEPENDENT RNA POLYMERASE=== |
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| ==Overview==
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| Various classes of nucleotidyl polymerases with different transcriptional roles contain a conserved core structure. Less is known, however, about the distinguishing features of these enzymes, particularly those of the RNA-dependent RNA polymerase class. The 1. 9 A resolution crystal structure of hepatitis C virus (HCV) nonstructural protein 5B (NS5B) presented here provides the first complete and detailed view of an RNA-dependent RNA polymerase. While canonical polymerase features exist in the structure, NS5B adopts a unique shape due to extensive interactions between the fingers and thumb polymerase subdomains that serve to encircle the enzyme active site. Several insertions in the fingers subdomain account for intersubdomain linkages that include two extended loops and a pair of antiparallel alpha-helices. The HCV NS5B apoenzyme structure reported here can accommodate a template:primer duplex without global conformational changes, supporting the hypothesis that this structure is essentially preserved during the reaction pathway. This NS5B template:primer model also allows identification of a new structural motif involved in stabilizing the nascent base pair.
| | The line below this paragraph, {{ABSTRACT_PUBMED_10504728}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 10504728 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_10504728}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Polymerase rna-dependent rna polymerase hepatitis c virus ns5b apoenzyme]] | | [[Category: Polymerase rna-dependent rna polymerase hepatitis c virus ns5b apoenzyme]] |
| [[Category: Transferase]] | | [[Category: Transferase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:15:43 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:07:39 2008'' |
