1c4l: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
Line 1: Line 1:
[[Image:1c4l.gif|left|200px]]
{{Seed}}
[[Image:1c4l.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1c4l|  PDB=1c4l  |  SCENE=  }}  
{{STRUCTURE_1c4l|  PDB=1c4l  |  SCENE=  }}  


'''SOLUTION STRUCTURE OF AN RNA DUPLEX INCLUDING A C-U BASE-PAIR'''
===SOLUTION STRUCTURE OF AN RNA DUPLEX INCLUDING A C-U BASE-PAIR===




==Overview==
<!--
The formation of the C-U base pair in a duplex was observed in solution by means of the temperature profile of (15)N chemical shifts, and the precise geometry of the C-U base pair was also determined by NOE-based structure calculation. From the solution structure of the RNA oligomer, r[CGACUCAGG].r[CCUGCGUCG], it was found that a single C-U mismatch preferred being stacked in the duplex rather than being flipped-out even in solution. Moreover, it adopts an irregular geometry, where the amino nitrogen (N4) of the cytidine and keto-oxygen (O4) of the uridine are within hydrogen-bonding distance, as seen in crystals. To further prove the presence of a hydrogen bond in the C-U pair, we employed a point-labeled cytidine at the exocyclic amino nitrogen of the cytidine in the C-U pair. The temperature profile of its (15)N chemical shift showed a sigmoidal transition curve, indicating the presence of a hydrogen bond in the C-U pair in the duplex.
The line below this paragraph, {{ABSTRACT_PUBMED_10852704}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 10852704 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_10852704}}


==About this Structure==
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C4L OCA].  
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C4L OCA].  


==Reference==
==Reference==
Line 31: Line 35:
[[Category: Yasuno, K.]]
[[Category: Yasuno, K.]]
[[Category: C-u base pair]]
[[Category: C-u base pair]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 12:19:17 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:12:03 2008''

Revision as of 17:12, 30 June 2008

File:1c4l.png

Template:STRUCTURE 1c4l

SOLUTION STRUCTURE OF AN RNA DUPLEX INCLUDING A C-U BASE-PAIR

Template:ABSTRACT PUBMED 10852704

About this Structure

Full experimental information is available from OCA.

Reference

Solution structure of an RNA duplex including a C-U base pair., Tanaka Y, Kojima C, Yamazaki T, Kodama TS, Yasuno K, Miyashita S, Ono A, Ono A, Kainosho M, Kyogoku Y, Biochemistry. 2000 Jun 20;39(24):7074-80. PMID:10852704

Page seeded by OCA on Mon Jun 30 20:12:03 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1c4l&oldid=580965"