1tlj: Difference between revisions

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<StructureSection load='1tlj' size='340' side='right'caption='[[1tlj]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
<StructureSection load='1tlj' size='340' side='right'caption='[[1tlj]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1tlj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1TLJ FirstGlance]. <br>
<table><tr><td colspan='2'>[[1tlj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TLJ FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SSO0622, C08_028 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 ATCC 35091])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1tlj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tlj OCA], [http://pdbe.org/1tlj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tlj RCSB], [http://www.ebi.ac.uk/pdbsum/1tlj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tlj ProSAT], [http://www.topsan.org/Proteins/MCSG/1tlj TOPSAN]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tlj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tlj OCA], [https://pdbe.org/1tlj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tlj RCSB], [https://www.ebi.ac.uk/pdbsum/1tlj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tlj ProSAT], [https://www.topsan.org/Proteins/MCSG/1tlj TOPSAN]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/TYW3_SULSO TYW3_SULSO]] S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wyosine derivatives biosynthesis pathway. Probably methylates N-4 position of wybutosine-86 to produce wybutosine-72.[HAMAP-Rule:MF_00266]  
[https://www.uniprot.org/uniprot/TYW3_SACS2 TYW3_SACS2] S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wyosine derivatives biosynthesis pathway. Probably methylates N-4 position of wybutosine-86 to produce wybutosine-72.[HAMAP-Rule:MF_00266]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tlj ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tlj ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
S-adenosylmethionine (SAM)-dependent methyltransferases regulate a wide range of biological processes through the modification of proteins, nucleic acids, polysaccharides, as well as various metabolites. TYW3/Taw3 is a SAM-dependent methyltransferase responsible for the formation of a tRNA modification known as wybutosine and its derivatives that are required for accurate decoding in protein synthesis. Here, we report the crystal structure of Taw3, a homolog of TYW3 from Sulfolobus solfataricus, which revealed a novel alpha/beta fold. The sequence motif (S/T)xSSCxGR and invariant aspartate and histidine, conserved in TYW3/Taw3, cluster to form the catalytic center. These structural and sequence features indicate that TYW3/Taw3 proteins constitute a distinct class of SAM-dependent methyltransferases. Using site-directed mutagenesis along with in vivo complementation assays combined with mass spectrometry as well as ligand docking and cofactor binding assays, we have identified the active site of TYW3 and residues essential for cofactor binding and methyltransferase activity.
Structural and functional characterization of the TYW3/Taw3 class of SAM-dependent methyltransferases.,Currie MA, Brown G, Wong A, Ohira T, Sugiyama K, Suzuki T, Yakunin AF, Jia Z RNA. 2017 Mar;23(3):346-354. doi: 10.1261/rna.057943.116. Epub 2016 Dec 8. PMID:27932585<ref>PMID:27932585</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1tlj" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc 35091]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Edwards, A]]
[[Category: Saccharolobus solfataricus]]
[[Category: Jia, Z]]
[[Category: Edwards A]]
[[Category: Kudrytska, M]]
[[Category: Jia Z]]
[[Category: Structural genomic]]
[[Category: Kudrytska M]]
[[Category: Savchenko, A]]
[[Category: Savchenko A]]
[[Category: Skarina, T]]
[[Category: Skarina T]]
[[Category: Walker, J]]
[[Category: Walker J]]
[[Category: Wong, A H.Y]]
[[Category: Wong AHY]]
[[Category: Mcsg]]
[[Category: PSI, Protein structure initiative]]
[[Category: Sso0622]]
[[Category: Unknown function]]
Retrieved from "https://proteopedia.org/1tlj"