6tqo: Difference between revisions

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====
==rrn anti-termination complex==
<StructureSection load='6tqo' size='340' side='right'caption='[[6tqo]]' scene=''>
<StructureSection load='6tqo' size='340' side='right'caption='[[6tqo]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
<table><tr><td colspan='2'>[[6tqo]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TQO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TQO FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tqo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tqo OCA], [http://pdbe.org/6tqo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tqo RCSB], [http://www.ebi.ac.uk/pdbsum/6tqo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tqo ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tqo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tqo OCA], [https://pdbe.org/6tqo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tqo RCSB], [https://www.ebi.ac.uk/pdbsum/6tqo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tqo ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SUHB_ECOLI SUHB_ECOLI]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacterial ribosomal RNAs are synthesized by a dedicated, conserved transcription-elongation complex that transcribes at high rates, shields RNA polymerase from premature termination, and supports co-transcriptional RNA folding, modification, processing, and ribosomal subunit assembly by presently unknown mechanisms. We have determined cryo-electron microscopy structures of complete Escherichia coli ribosomal RNA transcription elongation complexes, comprising RNA polymerase; DNA; RNA bearing an N-utilization-site-like anti-termination element; Nus factors A, B, E, and G; inositol mono-phosphatase SuhB; and ribosomal protein S4. Our structures and structure-informed functional analyses show that fast transcription and anti-termination involve suppression of NusA-stabilized pausing, enhancement of NusG-mediated anti-backtracking, sequestration of the NusG C-terminal domain from termination factor rho, and the rho blockade. Strikingly, the factors form a composite RNA chaperone around the RNA polymerase RNA-exit tunnel, which supports co-transcriptional RNA folding and annealing of distal RNA regions. Our work reveals a polymerase/chaperone machine required for biosynthesis of functional ribosomes.
Structure-Based Mechanisms of a Molecular RNA Polymerase/Chaperone Machine Required for Ribosome Biosynthesis.,Huang YH, Hilal T, Loll B, Burger J, Mielke T, Bottcher C, Said N, Wahl MC Mol Cell. 2020 Sep 17;79(6):1024-1036.e5. doi: 10.1016/j.molcel.2020.08.010. Epub, 2020 Aug 31. PMID:32871103<ref>PMID:32871103</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6tqo" style="background-color:#fffaf0;"></div>
==See Also==
*[[Inositol monophosphatase 3D structures|Inositol monophosphatase 3D structures]]
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Z-disk]]
[[Category: Hilal T]]
[[Category: Huang YH]]
[[Category: Loll B]]
[[Category: Said N]]
[[Category: Wahl MC]]
Retrieved from "https://proteopedia.org/6tqo"