1m5j: Difference between revisions

Line 1:

[[Image:1m5j.gif|left|200px]]<br />

[[Image:1m5j.gif|left|200px]]<br /><applet load="1m5j" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1m5j" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1m5j, resolution 2.40Å" />

'''CRYSTAL STRUCTURE OF CYANOVIRIN-N COMPLEXED TO A SYNTHETIC HEXAMANNOSIDE'''<br />

==Overview==

The development of anti-human immunodeficiency virus (HIV) microbicides, for either topical or ex vivo use is of considerable interest, mainly due, to the difficulties in creating a vaccine that would be active against, multiple clades of HIV. Cyanovirin-N (CV-N), an 11-kDa protein from the, cyanobacterium (blue-green algae) Nostoc ellipsosporum with potent, virucidal activity, was identified in the search for such antiviral, agents. The binding of CV-N to the heavily glycosylated HIV envelope, protein gp120 is carbohydrate-dependent. Since previous CV-N-dimannose, structures could not fully explain CV-N-oligomannose binding, we, determined the crystal structures of recombinant CV-N complexed to Man-9, and a synthetic hexamannoside, at 2.5- and 2.4-A resolution, respectively., CV-N is a three-dimensional domain-swapped dimer in the crystal structures, with two primary sites near the hinge region and two secondary sites on, the opposite ends of the dimer. The binding interface is constituted of, three stacked alpha1-->2-linked mannose rings for Man-9 and two stacked, mannose rings for hexamannoside with the rest of the saccharide molecules, pointing to the solution. These structures show unequivocally the binding, geometry of high mannose sugars to CV-N, permitting a better understanding, of carbohydrate binding to this potential new lead for the design of drugs, against AIDS.

The development of anti-human immunodeficiency virus (HIV) microbicides for either topical or ex vivo use is of considerable interest, mainly due to the difficulties in creating a vaccine that would be active against multiple clades of HIV. Cyanovirin-N (CV-N), an 11-kDa protein from the cyanobacterium (blue-green algae) Nostoc ellipsosporum with potent virucidal activity, was identified in the search for such antiviral agents. The binding of CV-N to the heavily glycosylated HIV envelope protein gp120 is carbohydrate-dependent. Since previous CV-N-dimannose structures could not fully explain CV-N-oligomannose binding, we determined the crystal structures of recombinant CV-N complexed to Man-9 and a synthetic hexamannoside, at 2.5- and 2.4-A resolution, respectively. CV-N is a three-dimensional domain-swapped dimer in the crystal structures with two primary sites near the hinge region and two secondary sites on the opposite ends of the dimer. The binding interface is constituted of three stacked alpha1-->2-linked mannose rings for Man-9 and two stacked mannose rings for hexamannoside with the rest of the saccharide molecules pointing to the solution. These structures show unequivocally the binding geometry of high mannose sugars to CV-N, permitting a better understanding of carbohydrate binding to this potential new lead for the design of drugs against AIDS.

==About this Structure==

1M5J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum] with NHE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1M5J OCA].

1M5J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum] with <scene name='pdbligand=NHE:'>NHE</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5J OCA].

==Reference==

Line 15:

Line 14:

[[Category: Single protein]]

[[Category: Botos, I.]]

[[Category: Boyd, M.R.]]

[[Category: Boyd, M R.]]

[[Category: Cartner, L.K.]]

[[Category: Cartner, L K.]]

[[Category: Keefe, B.R.O.]]

[[Category: Keefe, B R.O.]]

[[Category: Ratner, D.M.]]

[[Category: Ratner, D M.]]

[[Category: Seeberger, P.H.]]

[[Category: Seeberger, P H.]]

[[Category: Shenoy, S.R.]]

[[Category: Shenoy, S R.]]

[[Category: Wlodawer, A.]]

[[Category: NHE]]

Line 30:

Line 29:

[[Category: oligosaccharide]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on Thu ~~Nov 8 14~~:18:~~30 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:51:43 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1m5j.gif|left|200px]]<br />
+[[Image:1m5j.gif|left|200px]]<br /><applet load="1m5j" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1m5j" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1m5j, resolution 2.40&Aring;" />
 '''CRYSTAL STRUCTURE OF CYANOVIRIN-N COMPLEXED TO A SYNTHETIC HEXAMANNOSIDE'''<br />
 ==Overview==
-The development of anti-human immunodeficiency virus (HIV) microbicides, for either topical or ex vivo use is of considerable interest, mainly due, to the difficulties in creating a vaccine that would be active against, multiple clades of HIV. Cyanovirin-N (CV-N), an 11-kDa protein from the, cyanobacterium (blue-green algae) Nostoc ellipsosporum with potent, virucidal activity, was identified in the search for such antiviral, agents. The binding of CV-N to the heavily glycosylated HIV envelope, protein gp120 is carbohydrate-dependent. Since previous CV-N-dimannose, structures could not fully explain CV-N-oligomannose binding, we, determined the crystal structures of recombinant CV-N complexed to Man-9, and a synthetic hexamannoside, at 2.5- and 2.4-A resolution, respectively., CV-N is a three-dimensional domain-swapped dimer in the crystal structures, with two primary sites near the hinge region and two secondary sites on, the opposite ends of the dimer. The binding interface is constituted of, three stacked alpha1--&gt;2-linked mannose rings for Man-9 and two stacked, mannose rings for hexamannoside with the rest of the saccharide molecules, pointing to the solution. These structures show unequivocally the binding, geometry of high mannose sugars to CV-N, permitting a better understanding, of carbohydrate binding to this potential new lead for the design of drugs, against AIDS.
+The development of anti-human immunodeficiency virus (HIV) microbicides for either topical or ex vivo use is of considerable interest, mainly due to the difficulties in creating a vaccine that would be active against multiple clades of HIV. Cyanovirin-N (CV-N), an 11-kDa protein from the cyanobacterium (blue-green algae) Nostoc ellipsosporum with potent virucidal activity, was identified in the search for such antiviral agents. The binding of CV-N to the heavily glycosylated HIV envelope protein gp120 is carbohydrate-dependent. Since previous CV-N-dimannose structures could not fully explain CV-N-oligomannose binding, we determined the crystal structures of recombinant CV-N complexed to Man-9 and a synthetic hexamannoside, at 2.5- and 2.4-A resolution, respectively. CV-N is a three-dimensional domain-swapped dimer in the crystal structures with two primary sites near the hinge region and two secondary sites on the opposite ends of the dimer. The binding interface is constituted of three stacked alpha1--&gt;2-linked mannose rings for Man-9 and two stacked mannose rings for hexamannoside with the rest of the saccharide molecules pointing to the solution. These structures show unequivocally the binding geometry of high mannose sugars to CV-N, permitting a better understanding of carbohydrate binding to this potential new lead for the design of drugs against AIDS.
 ==About this Structure==
-M5J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum] with NHE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1M5J OCA].
+M5J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum] with <scene name='pdbligand=NHE:'>NHE</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M5J OCA].
 ==Reference==
@@ Line 15: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Botos, I.]]
-[[Category: Boyd, M.R.]]
+[[Category: Boyd, M R.]]
-[[Category: Cartner, L.K.]]
+[[Category: Cartner, L K.]]
-[[Category: Keefe, B.R.O.]]
+[[Category: Keefe, B R.O.]]
-[[Category: Ratner, D.M.]]
+[[Category: Ratner, D M.]]
-[[Category: Seeberger, P.H.]]
+[[Category: Seeberger, P H.]]
-[[Category: Shenoy, S.R.]]
+[[Category: Shenoy, S R.]]
 [[Category: Wlodawer, A.]]
 [[Category: NHE]]
@@ Line 30: / Line 29: @@
 [[Category: oligosaccharide]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov  8 14:18:30 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:51:43 2008''