6smt: Difference between revisions
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==S-enantioselective imine reductase from Mycobacterium smegmatis== | ==S-enantioselective imine reductase from Mycobacterium smegmatis== | ||
<StructureSection load='6smt' size='340' side='right'caption='[[6smt]]' scene=''> | <StructureSection load='6smt' size='340' side='right'caption='[[6smt]], [[Resolution|resolution]] 1.55Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SMT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SMT FirstGlance]. <br> | <table><tr><td colspan='2'>[[6smt]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SMT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SMT FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6smt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6smt OCA], [http://pdbe.org/6smt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6smt RCSB], [http://www.ebi.ac.uk/pdbsum/6smt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6smt ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2EH:(2S)-2-ETHYLHEXAN-1-OL'>2EH</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MSMEG_6341 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 MYCS2])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6smt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6smt OCA], [http://pdbe.org/6smt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6smt RCSB], [http://www.ebi.ac.uk/pdbsum/6smt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6smt ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
NADPH-dependent imine reductases (IREDs) are enzymes capable of enantioselectively reducing imines to chiral secondary amines, which represent important building blocks in the chemical and pharmaceutical industry. Since their discovery in 2011, many previously unknown IREDs have been identified, biochemically and structurally characterized and categorized into families. However, the catalytic mechanism and guiding principles for substrate specificity and stereoselectivity remain disputed. Herein, we describe the crystal structure of S-IRED-Ms from Mycobacterium smegmatis together with its cofactor NADPH. S-IRED-Ms belongs to the S-enantioselective superfamily 3 (SFam3) and is the first IRED from SFam3 to be structurally described. The data presented provide further evidence for the overall high degree of structural conservation between different IREDs of various superfamilies. We discuss the role of Asp170 in catalysis and the importance of hydrophobic amino acids in the active site for stereospecificity. Moreover, a separate entrance to the active site, potentially functioning according to a gatekeeping mechanism regulating access and, therefore, substrate specificity is described. | |||
Structural Characterization of an S-enantioselective Imine Reductase from Mycobacterium Smegmatis.,Meyer T, Zumbragel N, Geerds C, Groger H, Niemann HH Biomolecules. 2020 Jul 31;10(8). pii: biom10081130. doi: 10.3390/biom10081130. PMID:32751900<ref>PMID:32751900</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6smt" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Geerds C]] | [[Category: Mycs2]] | ||
[[Category: Groeger H]] | [[Category: Geerds, C]] | ||
[[Category: Meyer T]] | [[Category: Groeger, H]] | ||
[[Category: Niemann | [[Category: Meyer, T]] | ||
[[Category: Zumbraegel N]] | [[Category: Niemann, H H]] | ||
[[Category: Zumbraegel, N]] | |||
[[Category: Imine reductase]] | |||
[[Category: Oxidoreductase]] | |||
Revision as of 06:33, 19 August 2020
S-enantioselective imine reductase from Mycobacterium smegmatis
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