6smy: Difference between revisions
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==Crystal structure of SLA Reductase YihU from E. Coli with NADH and product DHPS== | ==Crystal structure of SLA Reductase YihU from E. Coli with NADH and product DHPS== | ||
<StructureSection load='6smy' size='340' side='right'caption='[[6smy]]' scene=''> | <StructureSection load='6smy' size='340' side='right'caption='[[6smy]], [[Resolution|resolution]] 2.45Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SMY OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[6smy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SMY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SMY FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LLQ:(2~{S})-2,3-bis(oxidanyl)propane-1-sulfonic+acid'>LLQ</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6smy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6smy OCA], [https://pdbe.org/6smy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6smy RCSB], [https://www.ebi.ac.uk/pdbsum/6smy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6smy ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/SQUU_ECOLI SQUU_ECOLI] Reduces 3-sulfolactaldehyde (SLA) to 2,3-dihydroxypropane 1-sulfonate (DHPS) (PubMed:24463506, Ref.6). Metabolite profiling studies showed that the enzyme also catalyzes in vitro the NADH-dependent reduction of succinic semialdehyde (SSA) to 4-hydroxybutyrate (GHB), and that it could be involved in the metabolism of SSA, and other potentially toxic intermediates that may accumulate under stress conditions (PubMed:19372223). However, the enzyme exhibits a 42,000-fold greater catalytic efficiency for the reduction of SLA over SSA (Ref.6). Shows no detectable activity on the analogous glycolytic intermediate glyceraldehyde-3-phosphate (Ref.6).<ref>PMID:19372223</ref> <ref>PMID:24463506</ref> <ref>PMID:24463506</ref> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Escherichia coli K-12]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Davies GJ]] | [[Category: Davies GJ]] | ||
[[Category: Sharma M]] | [[Category: Sharma M]] | ||
