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<StructureSection load='3csx' size='340' side='right'caption='[[3csx]], [[Resolution|resolution]] 1.84&Aring;' scene=''>
<StructureSection load='3csx' size='340' side='right'caption='[[3csx]], [[Resolution|resolution]] 1.84&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3csx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cyanothece Cyanothece]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CSX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3CSX FirstGlance]. <br>
<table><tr><td colspan='2'>[[3csx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Crocosphaera_subtropica_ATCC_51142 Crocosphaera subtropica ATCC 51142]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CSX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CSX FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.84&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3csx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3csx OCA], [http://pdbe.org/3csx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3csx RCSB], [http://www.ebi.ac.uk/pdbsum/3csx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3csx ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3csx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3csx OCA], [https://pdbe.org/3csx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3csx RCSB], [https://www.ebi.ac.uk/pdbsum/3csx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3csx ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A1KYE3_CROS5 A1KYE3_CROS5]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3csx ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3csx ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The genomes of many cyanobacteria contain the sequence for a small protein with a common "Domain of Unknown Function" grouped into the DUF683 protein family. While the biological function of DUF683 is still not known, their genomic location within nitrogen fixation clusters suggests that DUF683 proteins may play a role in the process. The diurnal cyanobacterium Cyanothece sp. PCC 51142 contains a gene for a protein that falls into the DUF683 family, cce_0567 (78 aa, 9.0 kDa). In an effort to elucidate the biochemical role DUF683 proteins may play in nitrogen fixation, we have determined the first crystal structure for a protein in this family, cce_0567, to 1.84 A resolution. Cce_0567 crystallized in space group P2(1) with two protein molecules and one Ni(2+) cation per asymmetric unit. The protein is composed of two alpha-helices, residues P11 to G41 (alpha1) and L49-E74 (alpha2), with the second alpha-helix containing a short 3(10)-helix (Y46-N48). A four-residue linker (L42-D45) between the helices allows them to form an anti-parallel bundle and cross over each other towards their termini. In solution it is likely that two molecules of cce_0567 form a rod-like dimer by the stacking interactions of approximately 1/2 of the protein. Histidine-36 is highly conserved in all known DUF683 proteins and the N2 nitrogen of the H36 side chain of each molecule in the dimer is coordinated with Ni(2+) in the crystal structure. The divalent cation Ni(2+) was titrated into (15)N-labeled cce_0567 and chemical shift perturbations were observed only in the (1)H-(15)N HSQC spectra for residues at, or near, the site of Ni(2+) binding observed in the crystal structure. There was no evidence for an increase in the size of cce_0567 upon binding Ni(2+), even in large molar excess of Ni(2+), indicating that a metal was not required for dimer formation. Circular dichroism spectroscopy indicated that cce_0567 was extremely robust, with a melting temperature of approximately 62 degrees C that was reversible.
Structural characterization of the protein cce_0567 from Cyanothece 51142, a metalloprotein associated with nitrogen fixation in the DUF683 family.,Buchko GW, Robinson H, Addlagatta A Biochim Biophys Acta. 2009 Apr;1794(4):627-33. Epub 2009 Jan 23. PMID:19336042<ref>PMID:19336042</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3csx" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Cyanothece]]
[[Category: Crocosphaera subtropica ATCC 51142]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Addlagatta, A]]
[[Category: Addlagatta A]]
[[Category: Buchko, G W]]
[[Category: Buchko GW]]
[[Category: Robinson, H]]
[[Category: Robinson H]]
[[Category: Circadian rhythm]]
[[Category: Cyanobacteria]]
[[Category: Metal binding protein]]
[[Category: Metalloprotein]]
[[Category: Nitrogen fixation]]
[[Category: Unknown function]]

Latest revision as of 09:38, 21 February 2024

Structural characterization of a protein in the DUF683 family- crystal structure of cce_0567 from the cyanobacterium Cyanothece 51142.

3csx, resolution 1.84Å

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