1ce2: Difference between revisions

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[[Image:1ce2.gif|left|200px]]
{{Seed}}
[[Image:1ce2.png|left|200px]]


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{{STRUCTURE_1ce2|  PDB=1ce2  |  SCENE=  }}  
{{STRUCTURE_1ce2|  PDB=1ce2  |  SCENE=  }}  


'''STRUCTURE OF DIFERRIC BUFFALO LACTOFERRIN AT 2.5A RESOLUTION'''
===STRUCTURE OF DIFERRIC BUFFALO LACTOFERRIN AT 2.5A RESOLUTION===




==Overview==
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The structure of buffalo lactoferrin has been determined at 303 K. The crystals belong to orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 77.5, b = 91.0, c = 131.5 A and Z = 4. The structure has been refined to an R factor of 0.187. The overall structure of the protein is similar to its structure determined at 277 K in a different crystal form. However, the lobe orientations in the two structures differ by 9.0 degrees, suggesting significant inter-lobe flexibility in this family of proteins. The inter-lobe interactions are predominantly hydrophobic and could act as a cushion for a change in orientation under the influence of external conditions. On the other hand, the domain arrangements are found to be similar in 277 and 303 K crystal structures, with orientations differing by 1.5 and 1.0 degrees in the N and C lobes, respectively. The results of these investigations suggest that the increase in temperature helps in the production of better quality crystals.
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{{ABSTRACT_PUBMED_10531476}}


==About this Structure==
==About this Structure==
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[[Category: Room temperature]]
[[Category: Room temperature]]
[[Category: Structure]]
[[Category: Structure]]
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