6sbe: Difference between revisions
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==Structure of type II terpene cyclase MstE_D109N from Scytonema in complex with geranylgeranyl dihydroxybenzoate (substrate)== | ==Structure of type II terpene cyclase MstE_D109N from Scytonema in complex with geranylgeranyl dihydroxybenzoate (substrate)== | ||
<StructureSection load='6sbe' size='340' side='right'caption='[[6sbe]]' scene=''> | <StructureSection load='6sbe' size='340' side='right'caption='[[6sbe]], [[Resolution|resolution]] 1.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SBE OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SBE FirstGlance]. <br> | <table><tr><td colspan='2'>[[6sbe]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Scytonema_sp._pcc_10023 Scytonema sp. pcc 10023]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SBE OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SBE FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sbe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sbe OCA], [http://pdbe.org/6sbe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sbe RCSB], [http://www.ebi.ac.uk/pdbsum/6sbe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sbe ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=L4E:geranylgeranyl+dihydroxybenzoate'>L4E</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6sbb|6sbb]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mstE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1680591 Scytonema sp. PCC 10023])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sbe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sbe OCA], [http://pdbe.org/6sbe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sbe RCSB], [http://www.ebi.ac.uk/pdbsum/6sbe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sbe ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Class II terpene cyclases, such as oxidosqualene and squalene-hopene cyclases, catalyse some of the most complex polycyclization reactions. They minimally exhibit a beta,gamma-didomain architecture that has been evolutionarily repurposed in a wide range of terpene-processing enzymes and likely resulted from a fusion of unidentified monodomain proteins. Although single domain class I terpene cyclases have already been identified, the corresponding class II counterparts have not been previously reported. Here we present high-resolution X-ray structures of a monodomain class II cyclase, merosterolic acid synthase (MstE). With a minimalistic beta-domain architecture, this cyanobacterial enzyme is able to construct four rings in cytotoxic meroterpenoids with a sterol-like topology. The structures with bound substrate, product, and inhibitor provide detailed snapshots of a cyclization mechanism largely governed by residues located in a noncanonical enzyme region. Our results complement the few known class II cyclase crystal structures, while also indicating that archaic monodomain cyclases might have already catalyzed complex reaction cascades. | |||
A monodomain class II terpene cyclase assembles complex isoprenoid scaffolds.,Moosmann P, Ecker F, Leopold-Messer S, Cahn JKB, Dieterich CL, Groll M, Piel J Nat Chem. 2020 Aug 10. pii: 10.1038/s41557-020-0515-3. doi:, 10.1038/s41557-020-0515-3. PMID:32778689<ref>PMID:32778689</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6sbe" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Cahn | [[Category: Scytonema sp. pcc 10023]] | ||
[[Category: Ecker F]] | [[Category: Cahn, J K.B]] | ||
[[Category: Groll M]] | [[Category: Ecker, F]] | ||
[[Category: Leopold-Messer S]] | [[Category: Groll, M]] | ||
[[Category: Moosmann P]] | [[Category: Leopold-Messer, S]] | ||
[[Category: Piel J]] | [[Category: Moosmann, P]] | ||
[[Category: Piel, J]] | |||
[[Category: Alpha6-alpha6 barrel]] | |||
[[Category: Biosynthetic protein]] | |||
[[Category: Marine drug]] | |||
[[Category: Merosterol]] | |||
[[Category: Type ii terpene cyclase]] | |||
