1cfm: Difference between revisions

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[[Image:1cfm.jpg|left|200px]]
{{Seed}}
[[Image:1cfm.png|left|200px]]


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{{STRUCTURE_1cfm|  PDB=1cfm  |  SCENE=  }}  
{{STRUCTURE_1cfm|  PDB=1cfm  |  SCENE=  }}  


'''CYTOCHROME F FROM CHLAMYDOMONAS REINHARDTII'''
===CYTOCHROME F FROM CHLAMYDOMONAS REINHARDTII===




==Overview==
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A truncated form of cytochrome f from Chlamydomonas reinhardtii (an important eukaryotic model organism for photosynthetic electron transfer studies) has been crystallized (space group P2(1)2(1)2(1); three molecules/asymmetric unit) and its structure determined to 2.0 A resolution by molecular replacement using the coordinates of a truncated turnip cytochrome f as a model. The structure displays the same folding and detailed features as turnip cytochrome f, including (a) an unusual heme Fe ligation by the alpha-amino group of tyrosine 1, (b) a cluster of lysine residues (proposed docking site of plastocyanin), and (c) the presence of a chain of seven water molecules bound to conserved residues and extending between the heme pocket and K58 and K66 at the lysine cluster. For this array of waters, we propose a structural role. Two cytochrome f molecules are related by a noncrystallographic symmetry operator which is a distorted proper 2-fold rotation. This may represent the dimeric relation of the monomers in situ; however, the heme orientation suggested by this model is not consistent with previous EPR measurements on oriented membranes.
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{{ABSTRACT_PUBMED_10869174}}


==About this Structure==
==About this Structure==
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[[Category: Plastocyanin]]
[[Category: Plastocyanin]]
[[Category: Proton wire]]
[[Category: Proton wire]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:41:14 2008''

Revision as of 17:41, 30 June 2008

File:1cfm.png

Template:STRUCTURE 1cfm

CYTOCHROME F FROM CHLAMYDOMONAS REINHARDTII

Template:ABSTRACT PUBMED 10869174

About this Structure

1CFM is a Single protein structure of sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA.

Reference

X-ray structure of a truncated form of cytochrome f from chlamydomonas reinhardtii., Chi YI, Huang LS, Zhang Z, Fernandez-Velasco JG, Berry EA, Biochemistry. 2000 Jul 4;39(26):7689-701. PMID:10869174

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