1cfl: Difference between revisions

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[[Image:1cfl.gif|left|200px]]
{{Seed}}
[[Image:1cfl.png|left|200px]]


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{{STRUCTURE_1cfl|  PDB=1cfl  |  SCENE=  }}  
{{STRUCTURE_1cfl|  PDB=1cfl  |  SCENE=  }}  


'''DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT'''
===DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT===




==Overview==
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The pyrimidine(6-4)pyrimidone photoproduct [(6-4) adduct] is one of the major photoproducts induced by UV irradiation of DNA and occurs at TpT sites. The (6-4) adduct is highly mutagenic and leads most often to a 3' T --&gt; C transition with 85% replicating error frequency [LeClerc, J. E., Borden, A. &amp; Lawrence, C. W. (1991) Proc. Natl. Acad. Sci. USA 88, 9685-9689]. To determine the origin of the specific 3' T --&gt; C transition of the (6-4) adduct, we have used experimental NMR restraints and molecular dynamics to determine the solution structure of a (6-4)-lesion DNA decamer duplex that contains a mismatched base pair between the 3' T residue and an opposed G residue. Normal Watson-Crick-type hydrogen bonding is retained at the 5' T of the lesion site. The O2 carbonyl of the 3' T residue forms hydrogen bonds with the imino and amino protons of the opposed G residue. This potential hydrogen bonding stabilizes the overall helix and restores the highly distorted conformation of the (6-4) adduct to the typical B-form-like DNA structure. This structural feature can explain the marked preference for the insertion of an A residue opposite the 5' T and a G residue opposite the 3' T of the (6-4) lesion during trans-lesion synthesis. Thus these insertions yield the predominant 3' T --&gt; C transition.
The line below this paragraph, {{ABSTRACT_PUBMED_10359763}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 10359763 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10359763}}


==About this Structure==
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFL OCA].  
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFL OCA].  


==Reference==
==Reference==
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[[Category: Mutagenesis]]
[[Category: Mutagenesis]]
[[Category: Nmr]]
[[Category: Nmr]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 12:41:01 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:41:00 2008''

Revision as of 17:41, 30 June 2008

File:1cfl.png

Template:STRUCTURE 1cfl

DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT

Template:ABSTRACT PUBMED 10359763

About this Structure

Full experimental information is available from OCA.

Reference

Solution structure of a DNA decamer duplex containing the stable 3' T.G base pair of the pyrimidine(6-4)pyrimidone photoproduct [(6-4) adduct]: implications for the highly specific 3' T --> C transition of the (6-4) adduct., Lee JH, Hwang GS, Choi BS, Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6632-6. PMID:10359763

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