Ire1: Difference between revisions
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==3D structures of Ire1== | ==3D structures of Ire1== | ||
[[2hz6]] – hIre1 | [[2hz6]], [[6shc]] – hIre1 luminal domain 24-419 (mutant) – human<br /> | ||
[[4z7g]], [[5hgi]] – hIre1 cytoplasmic domain <br /> | [[4z7g]], [[5hgi]] – hIre1 cytoplasmic domain 547-977<br /> | ||
[[3p23]], [[4yzd]] - hIre1 cytoplasmic domain + ADP<br /> | [[3p23]], [[4yzd]] - hIre1 cytoplasmic domain + ADP<br /> | ||
[[4u6r]], [[4yz9]], [[4z7h]] – hIre1 cytoplasmic domain + inhibitor <br /> | [[4u6r]], [[4yz9]], [[4z7h]], [[6urc]], [[6hv0]], [[6hx1]] – hIre1 cytoplasmic domain + inhibitor <br /> | ||
[[4yzc]] – hIre1 cytoplasmic domain + satuorosporine <br /> | [[4yzc]] – hIre1 cytoplasmic domain + satuorosporine <br /> | ||
[[4pl3]], [[4pl4]], [[4pl5]] – mIre1 cytoplasmic domain (mutant) + inhibitor – mouse<br /> | [[4pl3]], [[4pl4]], [[4pl5]] – mIre1 cytoplasmic domain (mutant) + inhibitor – mouse<br /> | ||
Revision as of 06:39, 26 August 2020
<StructureSection load='3lj0' size='400' side='right' caption='Structure of yeast Ire1 cytoplasmic domain dimer complex with quercetin, ADP, Ca+2 and Sr+2 ions (PDB entry 3lj0)' scene='51/516469/Cv/1'>
Function
Ire1 or serine/threonine protein kinase/endoribonuclease Ire1. It is important in altering gene expression as a response to endoplasmic reticulum-based stress signals[1]. The endoribonuclase domain of Ire1 is a transcriptional activator which triggers growth arrest and apoptosis. The kinase domain of Ire1 is required for activation of the endoribonuclase domain. Ire1 senses unfolded proteins causing its auto-activation.
Structural highlights
Yeast Ire1 structure contains 3 phosphorylated residues: 2 Ser and a Thr. Ire1 shows a different binding site for ADP and for quercetin. Quercetin is a powerful activator of Ire1. Ire1 binds 2 molecules of quercetin at its dimer interface[2].
3D structures of Ire1
2hz6, 6shc – hIre1 luminal domain 24-419 (mutant) – human
4z7g, 5hgi – hIre1 cytoplasmic domain 547-977
3p23, 4yzd - hIre1 cytoplasmic domain + ADP
4u6r, 4yz9, 4z7h, 6urc, 6hv0, 6hx1 – hIre1 cytoplasmic domain + inhibitor
4yzc – hIre1 cytoplasmic domain + satuorosporine
4pl3, 4pl4, 4pl5 – mIre1 cytoplasmic domain (mutant) + inhibitor – mouse
2rio, 3fbv, 3sdm – yIre1 cytoplasmic domain – yeast
3sdj - yIre1 cytoplasmic domain (mutant)
2bei – yIre1 lumenal domain + peptide
3lj0 - yIre1 cytoplasmic domain + quercetin + ADP
3lj1, 3lj2 - yIre1 cytoplasmic domain + inhibitor
References
- ↑ Urano F, Bertolotti A, Ron D. IRE1 and efferent signaling from the endoplasmic reticulum. J Cell Sci. 2000 Nov;113 Pt 21:3697-702. PMID:11034898
- ↑ Chen Y, Brandizzi F. IRE1: ER stress sensor and cell fate executor. Trends Cell Biol. 2013 Nov;23(11):547-55. doi: 10.1016/j.tcb.2013.06.005. Epub, 2013 Jul 21. PMID:23880584 doi:https://dx.doi.org/10.1016/j.tcb.2013.06.005
