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| [[Image:1ch9.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1ch9| PDB=1ch9 | SCENE= }} | | {{STRUCTURE_1ch9| PDB=1ch9 | SCENE= }} |
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| '''RECOMBINANT SPERM WHALE MYOGLOBIN H97Q MUTANT (MET)'''
| | ===RECOMBINANT SPERM WHALE MYOGLOBIN H97Q MUTANT (MET)=== |
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| ==Overview==
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| The ability of myoglobin to bind oxygen reversibly depends critically on retention of the heme prosthetic group. Globin side chains at the Leu(89)(F4), His(97)(FG3), Ile(99)(FG5), and Leu(104)(G5) positions on the proximal side of the heme pocket strongly influence heme affinity. The roles of these amino acids in preventing heme loss have been examined by determining high resolution structures of 14 different mutants at these positions using x-ray crystallography. Leu(89) and His(97) are important surface amino acids that interact either sterically or electrostatically with the edges of the porphyrin ring. Ile(99) and Leu(104) are located in the interior region of the proximal pocket beneath ring C of the heme prosthetic group. The apolar amino acids Leu(89), Ile(99), and Leu(104) "waterproof" the heme pocket by forming a barrier to solvent penetration, minimizing the size of the proximal cavity, and maintaining a hydrophobic environment. Substitutions with smaller or polar side chains at these positions result in exposure of the heme to solvent, the appearance of crystallographically defined water molecules in or near the proximal pocket, and large increases in the rate of hemin loss. Thus, the naturally occurring amino acid side chains at these positions serve to prevent hydration of the His(93)-Fe(III) bond and are highly conserved in all known myoglobins and hemoglobins. | | The line below this paragraph, {{ABSTRACT_PUBMED_11084036}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11084036 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11084036}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Muscle protein]] | | [[Category: Muscle protein]] |
| [[Category: Oxygen transport]] | | [[Category: Oxygen transport]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:44:02 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:44:40 2008'' |
