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New page: left|200px<br /> <applet load="1nj0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nj0" /> '''NMR structure of a V3 (MN isolate) peptide ...
 
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[[Image:1nj0.gif|left|200px]]<br />
[[Image:1nj0.gif|left|200px]]<br /><applet load="1nj0" size="350" color="white" frame="true" align="right" spinBox="true"  
<applet load="1nj0" size="450" color="white" frame="true" align="right" spinBox="true"  
caption="1nj0" />
caption="1nj0" />
'''NMR structure of a V3 (MN isolate) peptide bound to 447-52D, a human HIV-1 neutralizing antibody'''<br />
'''NMR structure of a V3 (MN isolate) peptide bound to 447-52D, a human HIV-1 neutralizing antibody'''<br />


==Overview==
==Overview==
The V3 loop of the HIV-1 envelope glycoprotein gp120 is involved in, binding to the CCR5 and CXCR4 coreceptors. The structure of an HIV-1(MN), V3 peptide bound to the Fv of the broadly neutralizing human monoclonal, antibody 447-52D was solved by NMR and found to be a beta hairpin. This, structure of V3(MN) was found to have conformation and sequence, similarities to beta hairpins in CD8 and CCR5 ligands MIP-1alpha, MIP-1beta, and RANTES and differed from the beta hairpin of a V3(IIIB), peptide bound to the strain-specific murine anti-gp120(IIIB) antibody, 0.5beta. In contrast to the structure of the bound V3(MN) peptide, the, V3(IIIB) peptide resembles a beta hairpin in SDF-1, a CXCR4 ligand. These, data suggest that the 447-52D-bound V3(MN) and the 0.5beta-bound V3(IIIB), structures represent alternative V3 conformations responsible for, selective interactions with CCR5 and CXCR4, respectively.
The V3 loop of the HIV-1 envelope glycoprotein gp120 is involved in binding to the CCR5 and CXCR4 coreceptors. The structure of an HIV-1(MN) V3 peptide bound to the Fv of the broadly neutralizing human monoclonal antibody 447-52D was solved by NMR and found to be a beta hairpin. This structure of V3(MN) was found to have conformation and sequence similarities to beta hairpins in CD8 and CCR5 ligands MIP-1alpha, MIP-1beta, and RANTES and differed from the beta hairpin of a V3(IIIB) peptide bound to the strain-specific murine anti-gp120(IIIB) antibody 0.5beta. In contrast to the structure of the bound V3(MN) peptide, the V3(IIIB) peptide resembles a beta hairpin in SDF-1, a CXCR4 ligand. These data suggest that the 447-52D-bound V3(MN) and the 0.5beta-bound V3(IIIB) structures represent alternative V3 conformations responsible for selective interactions with CCR5 and CXCR4, respectively.


==About this Structure==
==About this Structure==
1NJ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NJ0 OCA].  
1NJ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJ0 OCA].  


==Reference==
==Reference==
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[[Category: peptide-antibody complex]]
[[Category: peptide-antibody complex]]


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