5yn3

Crystal structure of xylose isomerase from Piromyces sp. E2

spinqualitylabelsall models 5yn3, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5yn3 is a 4 chain structure with sequence from Piromyces sp. E2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.7Å Ligands: GOL, MN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9P8C9_PIRSE Publication Abstract from PubMed Biofuel production using lignocellulosic biomass is gaining attention because it can be substituted for fossil fuels without competing with edible resources. However, because Saccharomyces cerevisiae does not have a D-xylose metabolic pathway, oxidoreductase or isomerase pathways must be introduced to utilize D-xylose from lignocellulosic biomass in S. cerevisiae. To elucidate the biochemical properties of xylose isomerase (XI) from Piromyces sp. E2 (PsXI), we determine its crystal structure in complex with substrate mimic glycerol. An amino acid sequence comparison with other reported XIs and the relative activity measurements using five kinds of divalent metal ions confirmed that PsXI belongs to class II XI. Moreover kinetic analysis of PsXI was also performed using Mn(2)(+), the preferred divalent metal ion for PsXI. In addition, the substrate-binding mode of PsXI could be predicted with the substrate mimic glycerol bound to the active site. These studies may provide structural information to enhance D-xylose utilization for biofuel production. Crystal Structure and Biochemical Characterization of Xylose Isomerase from Piromyces sp. E2.,Son H, Lee SM, Kim KJ J Microbiol Biotechnol. 2018 Apr 28;28(4):571-578. doi: 10.4014/jmb.1711.11026. PMID:29385668 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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