6cjc: Difference between revisions

Latest revision as of 15:05, 4 October 2023

CRYSTAL STRUCTURE OF A FC FRAGMENT LALA MUTANT (L234A, L235A) OF HUMAN IGG1 (CRYSTAL FORM 3)

Structural highlights

6cjc is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.575Å
Ligands:	BMA, CL, FUC, MAN, NAG
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q6MZV7_HUMAN

Publication Abstract from PubMed

Antibody structure couples adaptive and innate immunity via Fab (antigen binding) and Fc (effector) domains that are connected by unique hinge regions. Because antibodies harbor two or more Fab domains, they are capable of crosslinking multi-determinant antigens, which is required for Fc-dependent functions through associative interactions with effector ligands, including C1q and cell surface Fc receptors. The modular nature of antibodies, with distal ligand binding sites for antigen and Fc-ligands, is reminiscent of allosteric proteins, suggesting that allosteric interactions might contribute to Fc-mediated effector functions. This hypothesis has been pursued for over 40 years and remains unresolved. Here, we provide evidence that allosteric interactions between Fab and Fc triggered by antigen binding modulate binding of Fc to low-affinity Fc receptors (FcgammaR) for a human IgG1. This work opens the path to further dissection of the relative roles of allosteric and associative interactions in Fc-mediated effector functions.

Antigen-Induced Allosteric Changes in a Human IgG1 Fc Increase Low-Affinity Fcgamma Receptor Binding.,Orlandi C, Deredge D, Ray K, Gohain N, Tolbert W, DeVico AL, Wintrode P, Pazgier M, Lewis GK Structure. 2020 May 5;28(5):516-527.e5. doi: 10.1016/j.str.2020.03.001. Epub 2020, Mar 24. PMID:32209433^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Orlandi C, Deredge D, Ray K, Gohain N, Tolbert W, DeVico AL, Wintrode P, Pazgier M, Lewis GK. Antigen-Induced Allosteric Changes in a Human IgG1 Fc Increase Low-Affinity Fcgamma Receptor Binding. Structure. 2020 May 5;28(5):516-527.e5. doi: 10.1016/j.str.2020.03.001. Epub 2020, Mar 24. PMID:32209433 doi:https://dx.doi.org/10.1016/j.str.2020.03.001

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OCA

[1] Orlandi C, Deredge D, Ray K, Gohain N, Tolbert W, DeVico AL, Wintrode P, Pazgier M, Lewis GK. Antigen-Induced Allosteric Changes in a Human IgG1 Fc Increase Low-Affinity Fcgamma Receptor Binding. Structure. 2020 May 5;28(5):516-527.e5. doi: 10.1016/j.str.2020.03.001. Epub 2020, Mar 24. PMID:32209433 doi:https://dx.doi.org/10.1016/j.str.2020.03.001

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='6cjc' size='340' side='right'caption='[[6cjc]], [[Resolution|resolution]] 2.58&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6cjc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CJC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6CJC FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6cjc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CJC FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.575&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DKFZp686C11235 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6cjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cjc OCA], [http://pdbe.org/6cjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cjc RCSB], [http://www.ebi.ac.uk/pdbsum/6cjc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cjc ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cjc OCA], [https://pdbe.org/6cjc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cjc RCSB], [https://www.ebi.ac.uk/pdbsum/6cjc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cjc ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q6MZV7_HUMAN Q6MZV7_HUMAN]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Pazgier, M]]
+[[Category: Pazgier M]]
-[[Category: Tolbert, W D]]
+[[Category: Tolbert WD]]
-[[Category: Van, V]]
+[[Category: Van V]]
-[[Category: Fc fragment]]
-[[Category: Igg1]]
-[[Category: Immune system]]
-[[Category: Immunoglobulin]]
-[[Category: Immunoglobulin-like beta sandwich]]
-[[Category: Lala mutation]]

6cjc: Difference between revisions

Latest revision as of 15:05, 4 October 2023

CRYSTAL STRUCTURE OF A FC FRAGMENT LALA MUTANT (L234A, L235A) OF HUMAN IGG1 (CRYSTAL FORM 3)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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