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| [[Image:1cpm.gif|left|200px]] | | {{Seed}} |
| | [[Image:1cpm.png|left|200px]] |
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| {{STRUCTURE_1cpm| PDB=1cpm | SCENE= }} | | {{STRUCTURE_1cpm| PDB=1cpm | SCENE= }} |
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| '''NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS'''
| | ===NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS=== |
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| ==Overview==
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| A jellyroll beta-sandwich protein, the Bacillus beta-glucanase H(A16-M), is used to probe the role of N-terminal peptide regions in protein folding in vivo. A gene encoding H(A16-M) is rearranged to place residues 1-58 of the protein behind a signal peptide and residues 59-214. The rearranged gene is expressed in Escherichia coli. The resultant circularly permuted protein, cpA16M-59, is secreted into the periplasm, correctly processed, and folded into a stable and active enzyme. Crystal structure analysis at 2.0-A resolution, R = 15.3%, shows cpA16M-59 to have a three-dimensional structure nearly identical with that of the parent beta-glucanase. An analogous experiment based on the wild-type Bacillus macerans beta-glucanase, giving rise to the circularly permuted variant cpMAC-57, yields the same results. Folding of these proteins, therefore, is not a vectorial process depending on the conformation adopted by their native N-terminal oligopeptides after ribosomal synthesis and translocation through the cytoplasmic membrane.
| | The line below this paragraph, {{ABSTRACT_PUBMED_7937966}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 7937966 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_7937966}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Hahn, M.]] | | [[Category: Hahn, M.]] |
| [[Category: Heinemann, U.]] | | [[Category: Heinemann, U.]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:58:45 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 21:03:54 2008'' |
