5xav: Difference between revisions

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<StructureSection load='5xav' size='340' side='right'caption='[[5xav]], [[Resolution|resolution]] 1.48&Aring;' scene=''>
<StructureSection load='5xav' size='340' side='right'caption='[[5xav]], [[Resolution|resolution]] 1.48&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5xav]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XAV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XAV FirstGlance]. <br>
<table><tr><td colspan='2'>[[5xav]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chromobacterium_sp._USM2 Chromobacterium sp. USM2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XAV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XAV FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xav OCA], [http://pdbe.org/5xav PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xav RCSB], [http://www.ebi.ac.uk/pdbsum/5xav PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xav ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.479&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xav OCA], [https://pdbe.org/5xav PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xav RCSB], [https://www.ebi.ac.uk/pdbsum/5xav PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xav ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/E1APK1_9NEIS E1APK1_9NEIS]
Polyhydroxyalkanoate (PHA) is a promising candidate for use as an alternative bioplastic to replace petroleum-based plastics. Our understanding of PHA synthase PhaC is poor due to the paucity of available three-dimensional structural information. Here we present a high-resolution crystal structure of the catalytic domain of PhaC from Chromobacterium sp. USM2, PhaC Cs -CAT. The structure shows that PhaC Cs -CAT forms an alpha/beta hydrolase fold comprising alpha/beta core and CAP subdomains. The active site containing Cys291, Asp447 and His477 is located at the bottom of the cavity, which is filled with water molecules and is covered by the partly disordered CAP subdomain. We designated our structure as the closed form, which is distinct from the recently reported catalytic domain from Cupriavidus necator (PhaC Cn -CAT). Structural comparison showed PhaC Cn -CAT adopting a partially open form maintaining a narrow substrate access channel to the active site, but no product egress. PhaC Cs -CAT forms a face-to-face dimer mediated by the CAP subdomains. This arrangement of the dimer is also distinct from that of the PhaC Cn -CAT dimer. These findings suggest that the CAP subdomain should undergo a conformational change during catalytic activity that involves rearrangement of the dimer to facilitate substrate entry and product formation and egress from the active site.
 
Structure of polyhydroxyalkanoate (PHA) synthase PhaC from Chromobacterium sp. USM2, producing biodegradable plastics.,Chek MF, Kim SY, Mori T, Arsad H, Samian MR, Sudesh K, Hakoshima T Sci Rep. 2017 Jul 13;7(1):5312. doi: 10.1038/s41598-017-05509-4. PMID:28706283<ref>PMID:28706283</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5xav" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Chromobacterium sp. USM2]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Arsad, H]]
[[Category: Arsad H]]
[[Category: Chek, M F]]
[[Category: Chek MF]]
[[Category: Hakoshima, T]]
[[Category: Hakoshima T]]
[[Category: Kim, S Y]]
[[Category: Kim SY]]
[[Category: Mori, T]]
[[Category: Mori T]]
[[Category: Samian, M R]]
[[Category: Samian MR]]
[[Category: Sudesh, K]]
[[Category: Sudesh K]]
[[Category: Bioplastic synthase]]
[[Category: Biosynthetic protein]]
[[Category: Phac]]

Latest revision as of 10:17, 27 March 2024

Structure of PhaC from Chromobacterium sp. USM2

5xav, resolution 1.48Å

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