6xhh: Difference between revisions
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==Far-red absorbing dark state of JSC1_58120g3 with bound 18-1, 18-2 dihydrobiliverdin IXa (DHBV), the native chromophore precursor== | ==Far-red absorbing dark state of JSC1_58120g3 with bound 18-1, 18-2 dihydrobiliverdin IXa (DHBV), the native chromophore precursor== | ||
<StructureSection load='6xhh' size='340' side='right'caption='[[6xhh]]' scene=''> | <StructureSection load='6xhh' size='340' side='right'caption='[[6xhh]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XHH OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XHH FirstGlance]. <br> | <table><tr><td colspan='2'>[[6xhh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leptolyngbya_sp._jsc-1 Leptolyngbya sp. jsc-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XHH OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XHH FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xhh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xhh OCA], [http://pdbe.org/6xhh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xhh RCSB], [http://www.ebi.ac.uk/pdbsum/6xhh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xhh ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=M1V:MESOBILIVERDIN+IX(ALPHA)'>M1V</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xhh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xhh OCA], [http://pdbe.org/6xhh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xhh RCSB], [http://www.ebi.ac.uk/pdbsum/6xhh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xhh ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cyanobacteriochromes (CBCRs) are photoswitchable linear tetrapyrrole (bilin)-based light sensors in the phytochrome superfamily with a broad spectral range from the near UV through the far red (330 to 760 nm). The recent discovery of far-red absorbing CBCRs (frCBCRs) has garnered considerable interest from the optogenetic and imaging communities because of the deep penetrance of far-red light into mammalian tissue and the small size of the CBCR protein scaffold. The present studies were undertaken to determine the structural basis for far-red absorption by JSC1_58120g3, a frCBCR from the thermophilic cyanobacterium Leptolyngbya sp. JSC-1 that is a representative member of a phylogenetically distinct class. Unlike most CBCRs that bind phycocyanobilin (PCB), a phycobilin naturally occurring in cyanobacteria and only a few eukaryotic phototrophs, JSC1_58120g3's far-red absorption arises from incorporation of the PCB biosynthetic intermediate 18(1),18(2)-dihydrobiliverdin (18(1),18(2)-DHBV) rather than the more reduced and more abundant PCB. JSC1_58120g3 can also yield a far-red-absorbing adduct with the more widespread linear tetrapyrrole biliverdin IXalpha (BV), thus circumventing the need to coproduce or supplement optogenetic cell lines with PCB. Using high-resolution X-ray crystal structures of 18(1),18(2)-DHBV and BV adducts of JSC1_58120g3 along with structure-guided mutagenesis, we have defined residues critical for its verdin-binding preference and far-red absorption. Far-red sensing and verdin incorporation make this frCBCR lineage an attractive template for developing robust optogenetic and imaging reagents for deep tissue applications. | |||
A far-red cyanobacteriochrome lineage specific for verdins.,Moreno MV, Rockwell NC, Mora M, Fisher AJ, Lagarias JC Proc Natl Acad Sci U S A. 2020 Oct 26. pii: 2016047117. doi:, 10.1073/pnas.2016047117. PMID:33106421<ref>PMID:33106421</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6xhh" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Fisher | [[Category: Leptolyngbya sp. jsc-1]] | ||
[[Category: Lagarias | [[Category: Fisher, A J]] | ||
[[Category: Moreno | [[Category: Lagarias, J C]] | ||
[[Category: Rockwell | [[Category: Moreno, M V]] | ||
[[Category: Rockwell, N C]] | |||
[[Category: 18-2 dihydrobiliverdin]] | |||
[[Category: Cyanobacteriochrome gaf domain phytochrome-superfamily 18-1]] | |||
[[Category: Signaling protein]] | |||
