5z2v: Difference between revisions

Latest revision as of 08:44, 22 November 2023

Crystal structure of RecR from Pseudomonas aeruginosa PAO1

Structural highlights

5z2v is a 2 chain structure with sequence from Pseudomonas aeruginosa PAO1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Ligands:	GOL, N, NA, PO4, ZN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RECR_PSEAE May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO.

Publication Abstract from PubMed

DNA damage is usually lethal to all organisms. Homologous recombination plays an important role in the DNA damage-repair process in prokaryotic organisms. Two pathways are responsible for homologous recombination in Pseudomonas aeruginosa: the RecBCD pathway and the RecFOR pathway. RecR is an important regulator in the RecFOR homologous recombination pathway in P. aeruginosa. It forms complexes with RecF and RecO that can facilitate the loading of RecA onto ssDNA in the RecFOR pathway. Here, the crystal structure of RecR from P. aeruginosa PAO1 (PaRecR) is reported. PaRecR crystallizes in space group P6122, with two monomers per asymmetric unit. Analytical ultracentrifugation data show that PaRecR forms a stable dimer, but can exist as a tetramer in solution. The crystal structure shows that dimeric PaRecR forms a ring-like tetramer architecture via crystal symmetry. The presence of a ligand in the Walker B motif of one RecR subunit suggests a putative nucleotide-binding site.

Crystal structure of RecR, a member of the RecFOR DNA-repair pathway, from Pseudomonas aeruginosa PAO1.,Che S, Chen Y, Liang Y, Zhang Q, Bartlam M Acta Crystallogr F Struct Biol Commun. 2018 Apr 1;74(Pt 4):222-230. doi:, 10.1107/S2053230X18003503. Epub 2018 Mar 22. PMID:29633970^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Che S, Chen Y, Liang Y, Zhang Q, Bartlam M. Crystal structure of RecR, a member of the RecFOR DNA-repair pathway, from Pseudomonas aeruginosa PAO1. Acta Crystallogr F Struct Biol Commun. 2018 Apr 1;74(Pt 4):222-230. doi:, 10.1107/S2053230X18003503. Epub 2018 Mar 22. PMID:29633970 doi:https://dx.doi.org/10.1107/S2053230X18003503

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Che S, Chen Y, Liang Y, Zhang Q, Bartlam M. Crystal structure of RecR, a member of the RecFOR DNA-repair pathway, from Pseudomonas aeruginosa PAO1. Acta Crystallogr F Struct Biol Commun. 2018 Apr 1;74(Pt 4):222-230. doi:, 10.1107/S2053230X18003503. Epub 2018 Mar 22. PMID:29633970 doi:https://dx.doi.org/10.1107/S2053230X18003503

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='5z2v' size='340' side='right'caption='[[5z2v]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5z2v]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z2V OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Z2V FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5z2v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z2V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z2V FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=N:ANY+5-MONOPHOSPHATE+NUCLEOTIDE'>N</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5z2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z2v OCA], [http://pdbe.org/5z2v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z2v RCSB], [http://www.ebi.ac.uk/pdbsum/5z2v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z2v ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=N:ANY+5-MONOPHOSPHATE+NUCLEOTIDE'>N</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z2v OCA], [https://pdbe.org/5z2v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z2v RCSB], [https://www.ebi.ac.uk/pdbsum/5z2v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z2v ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/RECR_PSEAE RECR_PSEAE]] May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO.
+[https://www.uniprot.org/uniprot/RECR_PSEAE RECR_PSEAE] May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Bartlam, M]]
+[[Category: Pseudomonas aeruginosa PAO1]]
-[[Category: Che, S]]
+[[Category: Bartlam M]]
-[[Category: Zhang, Q]]
+[[Category: Che S]]
-[[Category: Dna binding protein]]
+[[Category: Zhang Q]]
-[[Category: Recr dna binding homologous recombination]]

5z2v: Difference between revisions

Latest revision as of 08:44, 22 November 2023

Crystal structure of RecR from Pseudomonas aeruginosa PAO1

Structural highlights

Function

Publication Abstract from PubMed

References

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