1cza: Difference between revisions

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[[Image:1cza.gif|left|200px]]
{{Seed}}
[[Image:1cza.png|left|200px]]


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{{STRUCTURE_1cza|  PDB=1cza  |  SCENE=  }}  
{{STRUCTURE_1cza|  PDB=1cza  |  SCENE=  }}  


'''MUTANT MONOMER OF RECOMBINANT HUMAN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE, GLUCOSE-6-PHOSPHATE, AND ADP'''
===MUTANT MONOMER OF RECOMBINANT HUMAN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE, GLUCOSE-6-PHOSPHATE, AND ADP===




==Overview==
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Hexokinase I, the pacemaker of glycolysis in brain tissue, is composed of two structurally similar halves connected by an alpha-helix. The enzyme dimerizes at elevated protein concentrations in solution and in crystal structures; however, almost all published data reflect the properties of a hexokinase I monomer in solution. Crystal structures of mutant forms of recombinant human hexokinase I, presented here, reveal the enzyme monomer for the first time. The mutant hexokinases bind both glucose 6-phosphate and glucose with high affinity to their N and C-terminal halves, and ADP, also with high affinity, to a site near the N terminus of the polypeptide chain. Exposure of the monomer crystals to ADP in the complete absence of glucose 6-phosphate reveals a second binding site for adenine nucleotides at the putative active site (C-half), with conformational changes extending 15 A to the contact interface between the N and C-halves. The structures reveal distinct conformational states for the C-half and a rigid-body rotation of the N-half, as possible elements of a structure-based mechanism for allosteric regulation of catalysis.
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{{ABSTRACT_PUBMED_10686099}}


==About this Structure==
==About this Structure==
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[[Category: Liu, X.]]
[[Category: Liu, X.]]
[[Category: Structurally homologous domain]]
[[Category: Structurally homologous domain]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 13:16:07 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 21:51:18 2008''

Revision as of 18:51, 30 June 2008

File:1cza.png


Drag the structure with the mouse to rotate
1cza, resolution 1.90Å (default scene)
Ligands: ADP, G6P, GLC
Activity: Hexokinase, with EC number 2.7.1.1
Related: 1hkb, 1hkc
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml



MUTANT MONOMER OF RECOMBINANT HUMAN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE, GLUCOSE-6-PHOSPHATE, AND ADP

Template:ABSTRACT PUBMED 10686099

About this Structure

1CZA is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structures of mutant monomeric hexokinase I reveal multiple ADP binding sites and conformational changes relevant to allosteric regulation., Aleshin AE, Kirby C, Liu X, Bourenkov GP, Bartunik HD, Fromm HJ, Honzatko RB, J Mol Biol. 2000 Mar 3;296(4):1001-15. PMID:10686099

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