6bi0

Trastuzumab Fab N158A, D185A, K190A (Light Chain) Triple Mutant.

spinqualitylabelsall models 6bi0, resolution 2.06Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6bi0 is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.057Å Ligands: EDO Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Activated esters are widely used to label proteins at lysine sidechains and N-termini. These reagents are useful for labeling virtually any protein, but robust reactivity toward primary amines generally precludes site-selective modification. In a unique case, fluoro-phenyl esters are shown to preferentially label human kappa antibodies at a single lysine (K188) within the light chain constant domain. Neighboring residues H189 and D151 contribute to the accelerated rate of labeling at K188 relative to ~40 other lysine sites. Enriched K188 labeling can be enhanced from 50-70% to >95% by any of these approaches: lowering reaction temperature, applying flow chemistry, or mutagenesis of specific residues in the surrounding protein environment. Our results demonstrate that activated esters with fluoro-substituted aromatic leaving groups, including a fluoro-napthyl ester, can be generally useful reagents for site-selective lysine labeling of antibodies and other immunoglobulin-type proteins. Tuning a Protein Labeling Reaction to Achieve Highly Site-Selective Lysine Conjugation.,Pham GH, Ou W, Bursulaya B, DiDonato M, Herath A, Jin Y, Hao X, Loren J, Spraggon G, Brock A, Uno T, Geierstanger BH, Cellitti S Chembiochem. 2018 Jan 31. doi: 10.1002/cbic.201700611. PMID:29388367 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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