6x8s: Difference between revisions

Revision as of 07:10, 3 April 2024

Crystal structure of 3D11 Fab in complex with Plasmodium berghei circumsporozoite protein NAND peptide

Structural highlights

6x8s is a 3 chain structure with sequence from Mus musculus and Plasmodium berghei ANKA. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.55Å
Ligands:	EDO
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CSP_PLABA Essential sporozoite protein (PubMed:6985745, PubMed:15630135, PubMed:16207248, PubMed:18005753, PubMed:21262960). In the mosquito vector, required for sporozoite development in the oocyst, migration through the vector hemolymph and entry into the vector salivary glands (PubMed:16207248, PubMed:21262960). In the vertebrate host, required for sporozoite migration through the host dermis and infection of host hepatocytes (PubMed:6985745, PubMed:15630135, PubMed:18005753, PubMed:21262960). Binds to highly sulfated heparan sulfate proteoglycans (HSPGs) on the surface of host hepatocytes (PubMed:18005753).^[1] ^[2] ^[3] ^[4] ^[5] In the vertebrate host, binds to highly sulfated heparan sulfate proteoglycans (HSPGs) on the surface of host hepatocytes and is required for sporozoite invasion of the host hepatocytes.^[6] ^[7]

References

↑ Coppi A, Pinzon-Ortiz C, Hutter C, Sinnis P. The Plasmodium circumsporozoite protein is proteolytically processed during cell invasion. J Exp Med. 2005 Jan 3;201(1):27-33. PMID:15630135 doi:10.1084/jem.20040989
↑ Wang Q, Fujioka H, Nussenzweig V. Mutational analysis of the GPI-anchor addition sequence from the circumsporozoite protein of Plasmodium. Cell Microbiol. 2005 Nov;7(11):1616-26. PMID:16207248 doi:10.1111/j.1462-5822.2005.00579.x
↑ Coppi A, Tewari R, Bishop JR, Bennett BL, Lawrence R, Esko JD, Billker O, Sinnis P. Heparan sulfate proteoglycans provide a signal to Plasmodium sporozoites to stop migrating and productively invade host cells. Cell Host Microbe. 2007 Nov 15;2(5):316-27. PMID:18005753 doi:10.1016/j.chom.2007.10.002
↑ Coppi A, Natarajan R, Pradel G, Bennett BL, James ER, Roggero MA, Corradin G, Persson C, Tewari R, Sinnis P. The malaria circumsporozoite protein has two functional domains, each with distinct roles as sporozoites journey from mosquito to mammalian host. J Exp Med. 2011 Feb 14;208(2):341-56. PMID:21262960 doi:10.1084/jem.20101488
↑ Yoshida N, Nussenzweig RS, Potocnjak P, Nussenzweig V, Aikawa M. Hybridoma produces protective antibodies directed against the sporozoite stage of malaria parasite. Science. 1980 Jan 4;207(4426):71-3. PMID:6985745 doi:10.1126/science.6985745
↑ Coppi A, Tewari R, Bishop JR, Bennett BL, Lawrence R, Esko JD, Billker O, Sinnis P. Heparan sulfate proteoglycans provide a signal to Plasmodium sporozoites to stop migrating and productively invade host cells. Cell Host Microbe. 2007 Nov 15;2(5):316-27. PMID:18005753 doi:10.1016/j.chom.2007.10.002
↑ Coppi A, Natarajan R, Pradel G, Bennett BL, James ER, Roggero MA, Corradin G, Persson C, Tewari R, Sinnis P. The malaria circumsporozoite protein has two functional domains, each with distinct roles as sporozoites journey from mosquito to mammalian host. J Exp Med. 2011 Feb 14;208(2):341-56. PMID:21262960 doi:10.1084/jem.20101488

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OCA

[1] Coppi A, Pinzon-Ortiz C, Hutter C, Sinnis P. The Plasmodium circumsporozoite protein is proteolytically processed during cell invasion. J Exp Med. 2005 Jan 3;201(1):27-33. PMID:15630135 doi:10.1084/jem.20040989

[2] Wang Q, Fujioka H, Nussenzweig V. Mutational analysis of the GPI-anchor addition sequence from the circumsporozoite protein of Plasmodium. Cell Microbiol. 2005 Nov;7(11):1616-26. PMID:16207248 doi:10.1111/j.1462-5822.2005.00579.x

[3] Coppi A, Tewari R, Bishop JR, Bennett BL, Lawrence R, Esko JD, Billker O, Sinnis P. Heparan sulfate proteoglycans provide a signal to Plasmodium sporozoites to stop migrating and productively invade host cells. Cell Host Microbe. 2007 Nov 15;2(5):316-27. PMID:18005753 doi:10.1016/j.chom.2007.10.002

[4] Coppi A, Natarajan R, Pradel G, Bennett BL, James ER, Roggero MA, Corradin G, Persson C, Tewari R, Sinnis P. The malaria circumsporozoite protein has two functional domains, each with distinct roles as sporozoites journey from mosquito to mammalian host. J Exp Med. 2011 Feb 14;208(2):341-56. PMID:21262960 doi:10.1084/jem.20101488

[5] Yoshida N, Nussenzweig RS, Potocnjak P, Nussenzweig V, Aikawa M. Hybridoma produces protective antibodies directed against the sporozoite stage of malaria parasite. Science. 1980 Jan 4;207(4426):71-3. PMID:6985745 doi:10.1126/science.6985745

[6] Coppi A, Tewari R, Bishop JR, Bennett BL, Lawrence R, Esko JD, Billker O, Sinnis P. Heparan sulfate proteoglycans provide a signal to Plasmodium sporozoites to stop migrating and productively invade host cells. Cell Host Microbe. 2007 Nov 15;2(5):316-27. PMID:18005753 doi:10.1016/j.chom.2007.10.002

[7] Coppi A, Natarajan R, Pradel G, Bennett BL, James ER, Roggero MA, Corradin G, Persson C, Tewari R, Sinnis P. The malaria circumsporozoite protein has two functional domains, each with distinct roles as sporozoites journey from mosquito to mammalian host. J Exp Med. 2011 Feb 14;208(2):341-56. PMID:21262960 doi:10.1084/jem.20101488

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@@ Line 1: / Line 1: @@
-====
+==Crystal structure of 3D11 Fab in complex with Plasmodium berghei circumsporozoite protein NAND peptide==
-<StructureSection load='6x8s' size='340' side='right'caption='[[6x8s]]' scene=''>
+<StructureSection load='6x8s' size='340' side='right'caption='[[6x8s]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6x8s]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Plasmodium_berghei_ANKA Plasmodium berghei ANKA]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X8S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X8S FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6x8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x8s OCA], [http://pdbe.org/6x8s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6x8s RCSB], [http://www.ebi.ac.uk/pdbsum/6x8s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6x8s ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x8s OCA], [https://pdbe.org/6x8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x8s RCSB], [https://www.ebi.ac.uk/pdbsum/6x8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x8s ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/CSP_PLABA CSP_PLABA] Essential sporozoite protein (PubMed:6985745, PubMed:15630135, PubMed:16207248, PubMed:18005753, PubMed:21262960). In the mosquito vector, required for sporozoite development in the oocyst, migration through the vector hemolymph and entry into the vector salivary glands (PubMed:16207248, PubMed:21262960). In the vertebrate host, required for sporozoite migration through the host dermis and infection of host hepatocytes (PubMed:6985745, PubMed:15630135, PubMed:18005753, PubMed:21262960). Binds to highly sulfated heparan sulfate proteoglycans (HSPGs) on the surface of host hepatocytes (PubMed:18005753).<ref>PMID:15630135</ref> <ref>PMID:16207248</ref> <ref>PMID:18005753</ref> <ref>PMID:21262960</ref> <ref>PMID:6985745</ref>   In the vertebrate host, binds to highly sulfated heparan sulfate proteoglycans (HSPGs) on the surface of host hepatocytes and is required for sporozoite invasion of the host hepatocytes.<ref>PMID:18005753</ref> <ref>PMID:21262960</ref>
+==See Also==
+*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
+== References ==
+<references/>
 __TOC__
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Z-disk]]
+[[Category: Mus musculus]]
+[[Category: Plasmodium berghei ANKA]]
+[[Category: Julien JP]]
+[[Category: Thai E]]

6x8s: Difference between revisions

Revision as of 07:10, 3 April 2024

Crystal structure of 3D11 Fab in complex with Plasmodium berghei circumsporozoite protein NAND peptide

Structural highlights

Function

See Also

References

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