2b1h: Difference between revisions

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[[Image:2b1h.gif|left|200px]]<br />

[[Image:2b1h.gif|left|200px]]<br /><applet load="2b1h" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="2b1h" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="2b1h, resolution 2.00Å" />

'''Crystal structure analysis of anti-HIV-1 V3 Fab 2219 in complex with UG29 peptide'''<br />

==Overview==

Human monoclonal antibody 2219 is a neutralizing antibody isolated from a, human immunodeficiency virus type 1-infected individual. 2219 was, originally selected for binding to a V3 fusion protein and can neutralize, primary isolates from subtypes B, A, and F. Thus, 2219 represents a, cross-reactive, human anti-V3 antibody. Fab 2219 binds to one face of the, variable V3 beta-hairpin, primarily contacting conserved residues on the, N-terminal beta-strand of V3, leaving the V3 crown or tip largely, accessible. Three V3/2219 complexes reveal the antibody-bound, conformations for both the N- and C-terminal regions that flank the V3, crown and illustrate how twisting of the V3 loop alters the relative, dispositions and pairing of the amino acids in the adjacent V3, beta-strands and how the antibody can accommodate V3 loops with different, sequences.

Human monoclonal antibody 2219 is a neutralizing antibody isolated from a human immunodeficiency virus type 1-infected individual. 2219 was originally selected for binding to a V3 fusion protein and can neutralize primary isolates from subtypes B, A, and F. Thus, 2219 represents a cross-reactive, human anti-V3 antibody. Fab 2219 binds to one face of the variable V3 beta-hairpin, primarily contacting conserved residues on the N-terminal beta-strand of V3, leaving the V3 crown or tip largely accessible. Three V3/2219 complexes reveal the antibody-bound conformations for both the N- and C-terminal regions that flank the V3 crown and illustrate how twisting of the V3 loop alters the relative dispositions and pairing of the amino acids in the adjacent V3 beta-strands and how the antibody can accommodate V3 loops with different sequences.

==About this Structure==

2B1H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2B1H OCA].

2B1H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B1H OCA].

==Reference==

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Line 13:

[[Category: Homo sapiens]]

[[Category: Single protein]]

[[Category: Gorny, M.K.]]

[[Category: Gorny, M K.]]

[[Category: Stanfield, R.L.]]

[[Category: Stanfield, R L.]]

[[Category: Wilson, I.A.]]

[[Category: Wilson, I A.]]

[[Category: Zolla-Pazner, S.]]

[[Category: fab-peptide complex; hiv-1; gp120; v3 loop]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on Thu ~~Nov 8 14~~:42:~~43 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:33:10 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2b1h.gif|left|200px]]<br />
+[[Image:2b1h.gif|left|200px]]<br /><applet load="2b1h" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="2b1h" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="2b1h, resolution 2.00&Aring;" />
 '''Crystal structure analysis of anti-HIV-1 V3 Fab 2219 in complex with UG29 peptide'''<br />
 ==Overview==
-Human monoclonal antibody 2219 is a neutralizing antibody isolated from a, human immunodeficiency virus type 1-infected individual. 2219 was, originally selected for binding to a V3 fusion protein and can neutralize, primary isolates from subtypes B, A, and F. Thus, 2219 represents a, cross-reactive, human anti-V3 antibody. Fab 2219 binds to one face of the, variable V3 beta-hairpin, primarily contacting conserved residues on the, N-terminal beta-strand of V3, leaving the V3 crown or tip largely, accessible. Three V3/2219 complexes reveal the antibody-bound, conformations for both the N- and C-terminal regions that flank the V3, crown and illustrate how twisting of the V3 loop alters the relative, dispositions and pairing of the amino acids in the adjacent V3, beta-strands and how the antibody can accommodate V3 loops with different, sequences.
+Human monoclonal antibody 2219 is a neutralizing antibody isolated from a human immunodeficiency virus type 1-infected individual. 2219 was originally selected for binding to a V3 fusion protein and can neutralize primary isolates from subtypes B, A, and F. Thus, 2219 represents a cross-reactive, human anti-V3 antibody. Fab 2219 binds to one face of the variable V3 beta-hairpin, primarily contacting conserved residues on the N-terminal beta-strand of V3, leaving the V3 crown or tip largely accessible. Three V3/2219 complexes reveal the antibody-bound conformations for both the N- and C-terminal regions that flank the V3 crown and illustrate how twisting of the V3 loop alters the relative dispositions and pairing of the amino acids in the adjacent V3 beta-strands and how the antibody can accommodate V3 loops with different sequences.
 ==About this Structure==
-B1H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2B1H OCA].
+B1H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B1H OCA].
 ==Reference==
@@ Line 14: / Line 13: @@
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
-[[Category: Gorny, M.K.]]
+[[Category: Gorny, M K.]]
-[[Category: Stanfield, R.L.]]
+[[Category: Stanfield, R L.]]
-[[Category: Wilson, I.A.]]
+[[Category: Wilson, I A.]]
 [[Category: Zolla-Pazner, S.]]
 [[Category: fab-peptide complex; hiv-1; gp120; v3 loop]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov  8 14:42:43 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:33:10 2008''