1d9e: Difference between revisions

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[[Image:1d9e.jpg|left|200px]]
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{{STRUCTURE_1d9e|  PDB=1d9e  |  SCENE=  }}  
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'''STRUCTURE OF E. COLI KDO8P SYNTHASE'''
===STRUCTURE OF E. COLI KDO8P SYNTHASE===




==Overview==
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3-deoxy-D-manno-octulosonate 8-phosphate (KDO8P) synthase catalyzes the condensation of phosphoenolpyruvate (PEP) with arabinose 5-phosphate (A5P) to form KDO8P and inorganic phosphate. KDO8P is the phosphorylated precursor of 3-deoxy-D-manno-octulosonate, an essential sugar of the lipopolysaccharide of Gram-negative bacteria. The crystal structure of the Escherichia coli KDO8P synthase has been determined by multiple wavelength anomalous diffraction and the model has been refined to 2.4 A (R-factor, 19.9%; R-free, 23.9%). KDO8P synthase is a homotetramer in which each monomer has the fold of a (beta/alpha)(8) barrel. On the basis of the features of the active site, PEP and A5P are predicted to bind with their phosphate moieties 13 A apart such that KDO8P synthesis would proceed via a linear intermediate. A reaction similar to KDO8P synthesis, the condensation of phosphoenolpyruvate, and erythrose 4-phosphate to form 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAH7P), is catalyzed by DAH7P synthase. In the active site of DAH7P synthase the two substrates PEP and erythrose 4-phosphate appear to bind in a configuration similar to that proposed for PEP and A5P in the active site of KDO8P synthase. This observation suggests that KDO8P synthase and DAH7P synthase evolved from a common ancestor and that they adopt the same catalytic strategy.
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{{ABSTRACT_PUBMED_10734095}}


==About this Structure==
==About this Structure==
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[[Category: Pep]]
[[Category: Pep]]
[[Category: Tim barrel]]
[[Category: Tim barrel]]
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Revision as of 19:42, 30 June 2008

File:1d9e.png

Template:STRUCTURE 1d9e

STRUCTURE OF E. COLI KDO8P SYNTHASE

Template:ABSTRACT PUBMED 10734095

About this Structure

1D9E is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure and mechanism of 3-deoxy-D-manno-octulosonate 8-phosphate synthase., Radaev S, Dastidar P, Patel M, Woodard RW, Gatti DL, J Biol Chem. 2000 Mar 31;275(13):9476-84. PMID:10734095

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