1dct: Difference between revisions

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[[Image:1dct.~~gif~~|left|200px]]

[[Image:1dct.png|left|200px]]

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~~'''~~DNA (CYTOSINE-5) METHYLASE FROM HAEIII COVALENTLY BOUND TO DNA~~'''~~

===DNA (CYTOSINE-5) METHYLASE FROM HAEIII COVALENTLY BOUND TO DNA===

~~==Overview==~~

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~~Many organisms expand the information content of their genome through enzymatic methylation of cytosine residues. Here we report the 2.8 A crystal structure of a bacterial DNA (cytosine~~-5)-~~methyltransferase (DCMtase)~~, ~~M. HaeIII~~, ~~bound covalently~~ to ~~DNA. In this complex,~~ the ~~substrate cytosine is extruded from the DNA helix and inserted into the active site of the enzyme,~~ as ~~has been observed for another DCMtase, M. HhaI. The DNA is bound in a cleft between the two domains of the protein and is distorted from the characteristic B-form conformation~~ at ~~its recognition sequence. A comparison of structures shows a variation in the mode of DNA recognition~~: M. ~~HaeIII differs from M~~. ~~HhaI in that the remaining bases in its recognition sequence undergo an extensive rearrangement in their pairing. In this process~~, the ~~bases are unstacked, and a gap 8 A long opens in the DNA~~.

The line below this paragraph, {{ABSTRACT_PUBMED_7606780}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 7606780 is the PubMed ID number.

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==About this Structure==

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[[Category: Cytosine methylase]]

[[Category: Enzyme]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May 2 13~~:42:13 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 22:50:12 2008''

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-[[Image:1dct.gif|left|200px]]
+{{Seed}}
+[[Image:1dct.png|left|200px]]
 <!--
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 {{STRUCTURE_1dct|  PDB=1dct  |  SCENE=  }}
-'''DNA (CYTOSINE-5) METHYLASE FROM HAEIII COVALENTLY BOUND TO DNA'''
+===DNA (CYTOSINE-5) METHYLASE FROM HAEIII COVALENTLY BOUND TO DNA===
-==Overview==
+<!--
-Many organisms expand the information content of their genome through enzymatic methylation of cytosine residues. Here we report the 2.8 A crystal structure of a bacterial DNA (cytosine-5)-methyltransferase (DCMtase), M. HaeIII, bound covalently to DNA. In this complex, the substrate cytosine is extruded from the DNA helix and inserted into the active site of the enzyme, as has been observed for another DCMtase, M. HhaI. The DNA is bound in a cleft between the two domains of the protein and is distorted from the characteristic B-form conformation at its recognition sequence. A comparison of structures shows a variation in the mode of DNA recognition: M. HaeIII differs from M. HhaI in that the remaining bases in its recognition sequence undergo an extensive rearrangement in their pairing. In this process, the bases are unstacked, and a gap 8 A long opens in the DNA.
+The line below this paragraph, {{ABSTRACT_PUBMED_7606780}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 7606780 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_7606780}}
 ==About this Structure==
@@ Line 29: / Line 33: @@
 [[Category: Cytosine methylase]]
 [[Category: Enzyme]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 13:42:13 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 22:50:12 2008''