5e4d

Hydroxynitrile lyase from the fern Davallia tyermanii in complex with benzoic acid

spinqualitylabelsall models 5e4d, resolution 1.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5e4d is a 2 chain structure with sequence from Davallia tyermanii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.85Å Ligands: BEZ Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A1C9V3S9_9MONI Publication Abstract from PubMed Homology and similarity based approaches are most widely used for the identification of new enzymes for biocatalysis. However, they are not suitable to find truly novel scaffolds with a desired function and this averts options and diversity. Hydroxynitrile lyases (HNLs) are an example of non-homologous isofunctional enzymes for the synthesis of chiral cyanohydrins. Due to their convergent evolution, finding new representatives is challenging. Here we show the discovery of unique HNL enzymes from the fern Davallia tyermannii by coalescence of transcriptomics, proteomics and enzymatic screening. It is the first protein with a Bet v1-like protein fold exhibiting HNL activity, and has a new catalytic center, as shown by protein crystallography. Biochemical properties of D. tyermannii HNLs open perspectives for the development of a complementary class of biocatalysts for the stereoselective synthesis of cyanohydrins. This work shows that systematic integration of -omics data facilitates discovery of enzymes with unpredictable sequences and helps to extend our knowledge about enzyme diversity. Enzyme discovery beyond homology: a unique hydroxynitrile lyase in the Bet v1 superfamily.,Lanfranchi E, Pavkov-Keller T, Koehler EM, Diepold M, Steiner K, Darnhofer B, Hartler J, Van Den Bergh T, Joosten HJ, Gruber-Khadjawi M, Thallinger GG, Birner-Gruenberger R, Gruber K, Winkler M, Glieder A Sci Rep. 2017 May 3;7:46738. doi: 10.1038/srep46738. PMID:28466867 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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