1dh3: Difference between revisions

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[[Image:1dh3.gif|left|200px]]
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[[Image:1dh3.png|left|200px]]


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{{STRUCTURE_1dh3|  PDB=1dh3  |  SCENE=  }}  
{{STRUCTURE_1dh3|  PDB=1dh3  |  SCENE=  }}  


'''CRYSTAL STRUCTURE OF A CREB BZIP-CRE COMPLEX REVEALS THE BASIS FOR CREB FAIMLY SELECTIVE DIMERIZATION AND DNA BINDING'''
===CRYSTAL STRUCTURE OF A CREB BZIP-CRE COMPLEX REVEALS THE BASIS FOR CREB FAIMLY SELECTIVE DIMERIZATION AND DNA BINDING===




==Overview==
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The cAMP responsive element-binding protein (CREB) is central to second messenger regulated transcription. To elucidate the structural mechanisms of DNA binding and selective dimerization of CREB, we determined to 3.0 A resolution, the structure of the CREB bZIP (residues 283-341) bound to a 21-base pair deoxynucleotide that encompasses the canonical 8-base pair somatostatin cAMP response element (SSCRE). The CREB dimer is stabilized in part by ionic interactions from Arg(314) to Glu(319') and Glu(328) to Lys(333') as well as a hydrogen bond network that links the carboxamide side chains of Gln(322')-Asn(321)-Asn(321')-Gln(322). Critical to family selective dimerization are intersubunit hydrogen bonds between basic region residue Tyr(307) and leucine zipper residue Glu(312), which are conserved in all CREB/CREM/ATF-1 family members. Strikingly, the structure reveals a hexahydrated Mg(2+) ion bound in the cavity between the basic region and SSCRE that makes a water-mediated DNA contact. DNA binding studies demonstrate that Mg(2+) ions enhance CREB bZIP:SSCRE binding by more than 25-fold and suggest a possible physiological role for this ion in somatostatin cAMP response element and potentially other CRE-mediated gene expression.
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{{ABSTRACT_PUBMED_10952992}}


==About this Structure==
==About this Structure==
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[[Category: Schumacher, M A.]]
[[Category: Schumacher, M A.]]
[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 13:51:01 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:04:04 2008''

Revision as of 20:04, 30 June 2008

File:1dh3.png

Template:STRUCTURE 1dh3

CRYSTAL STRUCTURE OF A CREB BZIP-CRE COMPLEX REVEALS THE BASIS FOR CREB FAIMLY SELECTIVE DIMERIZATION AND DNA BINDING

Template:ABSTRACT PUBMED 10952992

About this Structure

1DH3 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

The structure of a CREB bZIP.somatostatin CRE complex reveals the basis for selective dimerization and divalent cation-enhanced DNA binding., Schumacher MA, Goodman RH, Brennan RG, J Biol Chem. 2000 Nov 10;275(45):35242-7. PMID:10952992

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