7c2s: Difference between revisions

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<StructureSection load='7c2s' size='340' side='right'caption='[[7c2s]], [[Resolution|resolution]] 10.40&Aring;' scene=''>
<StructureSection load='7c2s' size='340' side='right'caption='[[7c2s]], [[Resolution|resolution]] 10.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7c2s]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C2S OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7C2S FirstGlance]. <br>
<table><tr><td colspan='2'>[[7c2s]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C2S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C2S FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7c2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c2s OCA], [http://pdbe.org/7c2s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7c2s RCSB], [http://www.ebi.ac.uk/pdbsum/7c2s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7c2s ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 10.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c2s OCA], [https://pdbe.org/7c2s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c2s RCSB], [https://www.ebi.ac.uk/pdbsum/7c2s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c2s ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/O39791_9FLAV O39791_9FLAV] Component of the viral RNA replication complex that functions in virion assembly and antagonizes the host immune response.[ARBA:ARBA00024317]  Functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter.[ARBA:ARBA00003504]  Serine protease subunit NS2B: Required cofactor for the serine protease function of NS3.[PROSITE-ProRule:PRU00859]
Previous flavivirus (dengue and Zika viruses) studies showed largely spherical particles either with smooth or bumpy surfaces. Here, we demonstrate flavivirus particles have high structural plasticity by the induction of a non-spherical morphology at elevated temperatures: the club-shaped particle (clubSP), which contains a cylindrical tail and a disc-like head. Complex formation of DENV and ZIKV with Fab C10 stabilize the viruses allowing cryoEM structural determination to ~10 A resolution. The caterpillar-shaped (catSP) Fab C10:ZIKV complex shows Fabs locking the E protein raft structure containing three E dimers. However, compared to the original spherical structure, the rafts have rotated relative to each other. The helical tail structure of Fab C10:DENV3 clubSP showed although the Fab locked an E protein dimer, the dimers have shifted laterally. Morphological diversity, including clubSP and the previously identified bumpy and smooth-surfaced spherical particles, may help flavivirus survival and immune evasion.


High flavivirus structural plasticity demonstrated by a non-spherical morphological variant.,Morrone SR, Chew VSY, Lim XN, Ng TS, Kostyuchenko VA, Zhang S, Wirawan M, Chew PL, Lee J, Tan JL, Wang J, Tan TY, Shi J, Screaton G, Morais MC, Lok SM Nat Commun. 2020 Jun 19;11(1):3112. doi: 10.1038/s41467-020-16925-y. PMID:32561757<ref>PMID:32561757</ref>
==See Also==
 
*[[Antibody 3D structures|Antibody 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7c2s" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Dengue virus 3]]
[[Category: Dengue virus 3]]
[[Category: Human]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Chew, S V]]
[[Category: Chew SV]]
[[Category: Kostyuchenko, V A]]
[[Category: Kostyuchenko VA]]
[[Category: Lim, X N]]
[[Category: Lim XN]]
[[Category: Lok, S M]]
[[Category: Lok SM]]
[[Category: Morrone, S]]
[[Category: Morrone S]]
[[Category: Ng, T S]]
[[Category: Ng TS]]
[[Category: Zhang, S]]
[[Category: Zhang S]]
[[Category: Antibody]]
[[Category: Neutralization]]
[[Category: Virus]]

Revision as of 10:50, 27 March 2024

Helical reconstruction of Dengue virus serotype 3 complexed with Fab C10

7c2s, resolution 10.40Å

Drag the structure with the mouse to rotate

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