Sandbox Reserved 1656: Difference between revisions
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==== Localization ==== | ==== Localization ==== | ||
The localization depends on the DUB we consider. However, the majority of DUBs are found in the nucleus, plasma membrane or/and in secretory and endocytic pathways. For example, in the ubiquitin-specific proteases family, the USP21 is mostly associated with microtubules and the centrosome. Thus this deubiquitinase depends on all the physiological mechanisms involving the [https:// | The localization depends on the DUB we consider. However, the majority of DUBs are found in the nucleus, plasma membrane or/and in secretory and endocytic pathways. For example, in the ubiquitin-specific proteases family, the USP21 is mostly associated with microtubules and the centrosome. Thus this deubiquitinase depends on all the physiological mechanisms involving the [https://en.wikipedia.org/wiki/Microtubule microtubules]. <ref>PMID:22298430</ref> | ||
== Structure == | == Structure == | ||
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==== The overall structure ==== | ==== The overall structure ==== | ||
3TMP is the catalytic domain of human deubiquitinase DUBA in complex with ubiquitin aldehyde. It is a 8 chain structure with sequence from Human. Indeed, 3TMP is made of these two macromolecules : OTU domain-containing protein 5 ( also named DUBA or OTUD5) and Polyubiquitin-C, which is the ubiquitin aldehyde. It is also made of two small molecules which are the phosphoserine (SEP) and the amino-acetaldehyde (GLZ), they are L-peptide links. <ref>PMID:22245969</ref> | 3TMP is the catalytic domain of human deubiquitinase DUBA in complex with ubiquitin aldehyde. It is a 8 chain structure with sequence from Human. Indeed, 3TMP is made of these two macromolecules : OTU domain-containing protein 5 (also named DUBA or OTUD5) and Polyubiquitin-C, which is the ubiquitin aldehyde. It is also made of two small molecules which are the phosphoserine (SEP) and the amino-acetaldehyde (GLZ), they are L-peptide links. <ref>PMID:22245969</ref> | ||
=== Impact of phosphorylation on DUB activity === | === Impact of phosphorylation on DUB activity === | ||
