2a3v: Difference between revisions

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<StructureSection load='2a3v' size='340' side='right'caption='[[2a3v]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
<StructureSection load='2a3v' size='340' side='right'caption='[[2a3v]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2a3v]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibch Vibch]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A3V FirstGlance]. <br>
<table><tr><td colspan='2'>[[2a3v]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A3V FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">intI4 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243277 VIBCH])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a3v OCA], [https://pdbe.org/2a3v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a3v RCSB], [https://www.ebi.ac.uk/pdbsum/2a3v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a3v ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a3v OCA], [https://pdbe.org/2a3v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a3v RCSB], [https://www.ebi.ac.uk/pdbsum/2a3v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a3v ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O68847_VIBCL O68847_VIBCL]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a3v ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a3v ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lateral DNA transfer--the movement of genetic traits between bacteria--has a profound impact on genomic evolution and speciation. The efficiency with which bacteria incorporate genetic information reflects their capacity to adapt to changing environmental conditions. Integron integrases are proteins that mediate site-specific DNA recombination between a proximal primary site (attI) and a secondary target site (attC) found within mobile gene cassettes encoding resistance or virulence factors. The lack of sequence conservation among attC sites has led to the hypothesis that a sequence-independent structural recognition determinant must exist within attC. Here we report the crystal structure of an integron integrase bound to an attC substrate. The structure shows that DNA target site recognition and high-order synaptic assembly are not dependent on canonical DNA but on the position of two flipped-out bases that interact in cis and in trans with the integrase. These extrahelical bases, one of which is required for recombination in vivo, originate from folding of the bottom strand of attC owing to its imperfect internal dyad symmetry. The mechanism reported here supports a new paradigm for how sequence-degenerate single-stranded genetic material is recognized and exchanged between bacteria.
Structural basis for broad DNA-specificity in integron recombination.,MacDonald D, Demarre G, Bouvier M, Mazel D, Gopaul DN Nature. 2006 Apr 27;440(7088):1157-62. PMID:16641988<ref>PMID:16641988</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2a3v" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Vibch]]
[[Category: Vibrio cholerae O1 biovar El Tor str. N16961]]
[[Category: Bouvier, M]]
[[Category: Bouvier M]]
[[Category: Demarre, G]]
[[Category: Demarre G]]
[[Category: Gopaul, D N]]
[[Category: Gopaul DN]]
[[Category: MacDonald, D]]
[[Category: MacDonald D]]
[[Category: Mazel, D]]
[[Category: Mazel D]]
[[Category: Protein-dna complex]]
[[Category: Recombination]]

Latest revision as of 09:11, 14 February 2024

Structural basis for broad DNA-specificity in integron recombination

2a3v, resolution 2.80Å

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