2ar8: Difference between revisions

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<StructureSection load='2ar8' size='340' side='right'caption='[[2ar8]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='2ar8' size='340' side='right'caption='[[2ar8]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2ar8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_fluorescens_liquefaciens"_flugge_1886 "bacillus fluorescens liquefaciens" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AR8 FirstGlance]. <br>
<table><tr><td colspan='2'>[[2ar8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AR8 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CTE:7-CHLOROTRYPTOPHAN'>CTE</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2apg|2apg]], [[2aqj|2aqj]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CTE:7-CHLOROTRYPTOPHAN'>CTE</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PrnA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 "Bacillus fluorescens liquefaciens" Flugge 1886])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ar8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ar8 OCA], [https://pdbe.org/2ar8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ar8 RCSB], [https://www.ebi.ac.uk/pdbsum/2ar8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ar8 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ar8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ar8 OCA], [https://pdbe.org/2ar8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ar8 RCSB], [https://www.ebi.ac.uk/pdbsum/2ar8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ar8 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/PRNA_PSEFL PRNA_PSEFL]] Involved in the biosynthesis of the antifungal antibiotic pyrrolnitrin. Catalyze the chlorination of tryptophan (Trp) at C7 position to yield 7-chloro-L-tryptophan (7-CLT). The reaction between FADH2, Cl-, and O2 generates the powerful oxidant HOCl, which is presumed to carry out the chlorination reaction. The reaction of HOCl with the active site Lys-79 generates a lysine chloramine, which plays a key role in directing regiospecific chlorination of substrate in this important class of biosynthetic enzymes. It is also able to use bromide ions to generate monobrominated Trp.<ref>PMID:10941070</ref> <ref>PMID:9172332</ref> <ref>PMID:9537395</ref>
[https://www.uniprot.org/uniprot/PRNA_PSEFL PRNA_PSEFL] Involved in the biosynthesis of the antifungal antibiotic pyrrolnitrin. Catalyze the chlorination of tryptophan (Trp) at C7 position to yield 7-chloro-L-tryptophan (7-CLT). The reaction between FADH2, Cl-, and O2 generates the powerful oxidant HOCl, which is presumed to carry out the chlorination reaction. The reaction of HOCl with the active site Lys-79 generates a lysine chloramine, which plays a key role in directing regiospecific chlorination of substrate in this important class of biosynthetic enzymes. It is also able to use bromide ions to generate monobrominated Trp.<ref>PMID:10941070</ref> <ref>PMID:9172332</ref> <ref>PMID:9537395</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bacillus fluorescens liquefaciens flugge 1886]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Dong, C]]
[[Category: Pseudomonas fluorescens]]
[[Category: Flecks, S]]
[[Category: Dong C]]
[[Category: Haupt, C]]
[[Category: Flecks S]]
[[Category: Naismith, J H]]
[[Category: Haupt C]]
[[Category: Pee, K H.Van]]
[[Category: Naismith JH]]
[[Category: SSPF, Scottish Structural Proteomics Facility]]
[[Category: Unversucht S]]
[[Category: Unversucht, S]]
[[Category: Van Pee KH]]
[[Category: Biosynthetic protein]]
[[Category: Flavin-dependent halogenase]]
[[Category: Helical bundle]]
[[Category: Sandwiched sheet]]
[[Category: Scottish structural proteomics facility]]
[[Category: Sspf]]
[[Category: Structural genomic]]
[[Category: Tryptophan 7-halogenase]]
Retrieved from "https://proteopedia.org/2ar8"