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| <StructureSection load='2ayg' size='340' side='right' caption='Human papillomavirus 6a regulatory protein E2 complex with DNA (PDB code [[2ayg]])' scene=''> | | <StructureSection load='2gxa' size='340' side='right' caption='Bovine papillomavirus 1 replication protein E1 complex with DNA (PDB code [[2gxa]])' scene=''> |
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| ==Function== | | ==Function== |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} |
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| Domains: transactivation (TAD) 2-201; DNA-binding (DBD) 286-365 | | Domains: helicase 301-605; DNA-binding (DBD) 286-365 |
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| [[1r8h]], [[2aye]] - RPE2 DBD - HPV6a<br /> | | [[1r9w]] - RPE1 DBD - HPV18<br /> |
| [[2ayb]], [[2ayg]] - RPE2 DBD + DNA<br />
| | [[1tue]] - RPE1 428-631 (mutant) + RPE2 TAD<br /> |
| [[1r6k]], [[1r6n]] - RPE2 TAD + inhibitor - HPV11<br />
| | [[2v9p]] - RPE1 helicase domain - BPV1<br /> |
| [[1dto]] - RPE2 TAD - HPV16<br />
| | [[5a9k]] - RPE1 helicase domain - Cryo EM<br /> |
| [[2nnu]] - RPE2 TAD + BRD4 peptide<br />
| | [[2gxa]] - RPE1 helicase domain + DNA + ADP<br /> |
| [[1by9]], [[2q79]] - RPE2 DBD<br />
| | [[1f08]] - RPE1 DBD<br /> |
| [[3mi7]] - RPE2 DBD (mutant)<br />
| | [[1ksx]], [[1ksy]] - RPE1 DBD + DNA<br /> |
| [[1r8p]], [[1zzf]] - RPE2 DBD - NMR<br />
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| [[1tue]] - RPE1 428-631 (mutant) + RPE2 TAD - HPV18<br /> | |
| [[1qqh]] - RPE2 TAD<br /> | |
| [[1f9f]] - RPE2 DBD<br /> | |
| [[1jj4]] - RPE2 DBD + DNA<br /> | |
| [[1dhm]] - RPE2 DBD - HPV31 - NMR<br /> | |
| [[1a7g]] - RPE2 DBD - HPV32<br /> | |
| [[2jeu]], [[2jex]] - RPE2 TAD (mutant) - BPV1<br />
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| [[1jjh]], [[6bus]] - RPE2 DBD<br />
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| [[1dbd]] - RPE2 DBD - NMR<br />
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| [[2bop]] - RPE2 DBD + DNA<br />
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| == References == | | == References == |
| <references/> | | <references/> |
| [[Category:Topic Page]] | | [[Category:Topic Page]] |
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Function
Replication protein E1 (RPE1) is a human papillomavirus (HPV) essential protein which is required for its replication. RPE1 recognizes the HPV origin of replication (ori) with the aid of regulatory protein E2 (RPE2). The resultant helicase complex initiates DNA unwinding to provide the template of progeny DNA synthesis, The HPV ori contains one RPE1 binding site and 2 RPE2 binding sites[1]. RPE2 is found also in bovine papillomavirus (BPV).
Disease
RPE2 induces apoptosis of its host cells[2].
Structural highlights
The complex between HPV6a DBD and 18-bp DNA (PDB code 2ayg) shows the binding of 2 recognition helices of the dimer to 2 successive major grooves of the target DNA making numerous contacts to the DNA backbone phosphate atoms and to its bases[3].
- ↑ Wilson VG, West M, Woytek K, Rangasamy D. Papillomavirus E1 proteins: form, function, and features. Virus Genes. 2002 Jun;24(3):275-90. doi: 10.1023/a:1015336817836. PMID:12086149 doi:https://dx.doi.org/10.1023/a:1015336817836
- ↑ Singh N, Senapati S, Bose K. Insights into the mechanism of human papillomavirus E2-induced procaspase-8 activation and cell death. Sci Rep. 2016 Feb 24;6:21408. doi: 10.1038/srep21408. PMID:26906543 doi:https://dx.doi.org/10.1038/srep21408
- ↑ Hooley E, Fairweather V, Clarke AR, Gaston K, Brady RL. The recognition of local DNA conformation by the human papillomavirus type 6 E2 protein. Nucleic Acids Res. 2006;34(14):3897-908. Epub 2006 Aug 12. PMID:16914454 doi:10.1093/nar/gkl466
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3D structures of regulatory protein E2
Updated on 27-January-2021
Domains: helicase 301-605; DNA-binding (DBD) 286-365
2ayg - RPE1 DBD - HPV18
1tue - RPE1 428-631 (mutant) + RPE2 TAD
2v9p - RPE1 helicase domain - BPV1
5a9k - RPE1 helicase domain - Cryo EM
2gxa - RPE1 helicase domain + DNA + ADP
1f08 - RPE1 DBD
1ksx, 1ksy - RPE1 DBD + DNA
References
