Replication protein E1: Difference between revisions

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==Function==

'''Replication protein E1''' (RPE1) is a human papillomavirus (HPV) essential protein which is required for its replication. RPE1 recognizes the HPV origin of replication (ori) with the aid of '''regulatory protein E2''' (RPE2). The resultant helicase complex initiates DNA unwinding to provide the template of progeny DNA synthesis, The HPV ori contains one RPE1 binding site and 2 RPE2 binding sites<ref>PMID:12086149</ref>. ~~RPE2~~ is found also in bovine papillomavirus (BPV).

'''Replication protein E1''' (RPE1) is a human papillomavirus (HPV) essential protein which is required for its replication. RPE1 recognizes the HPV origin of replication (ori) with the aid of '''regulatory protein E2''' (RPE2). The resultant helicase complex initiates DNA unwinding to provide the template of progeny DNA synthesis, The HPV ori contains one RPE1 binding site and 2 RPE2 binding sites<ref>PMID:12086149</ref>. RPE1 is found also in bovine papillomavirus (BPV).

== Disease ==

~~RPE2~~ induces apoptosis of its host cells<ref>PMID:~~26906543~~</ref>.

RPE1 has been shown to mitigate host cell defenses leading to the development of cancer induces apoptosis of its host cells<ref>PMID:29495005</ref>.

==Relevance==

The detection of RPE1 mRNA and promoter methylation may serve as cancer prognostic marker.

== Structural highlights ==

The complex between ~~HPV6a~~ DBD and 18-bp DNA (PDB code [[~~2ayg~~]]) shows ~~the~~ binding ~~of 2 recognition helices of the dimer to 2 successive major grooves of the target~~ DNA ~~making numerous contacts~~ to ~~the DNA backbone~~ phosphate atoms and ~~to its bases~~<ref>PMID:~~16914454~~</ref>.

The complex between BPV1 DBD and 21-bp DNA (PDB code [[1ksx]]) shows 2 DBD-DNA binding modules: a binding loop and a binding helix each interacting with a single strand. The protein-DNA interactions are hydrophilic to phosphate atoms and van der Waals hydrophobic ones<ref>PMID:11889054</ref>.

</StructureSection>

==3D structures of replication protein E1==

Replication protein E1: Difference between revisions

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@@ Line 3: / Line 3: @@
 ==Function==
-'''Replication protein E1''' (RPE1) is a human papillomavirus (HPV) essential protein which is required for its replication.  RPE1 recognizes the HPV origin of replication (ori) with the aid of '''regulatory protein E2''' (RPE2).  The resultant helicase complex initiates DNA unwinding to provide the template of progeny DNA synthesis,  The HPV ori contains one RPE1 binding site and 2 RPE2 binding sites<ref>PMID:12086149</ref>.  RPE2 is found also in bovine papillomavirus (BPV).
+'''Replication protein E1''' (RPE1) is a human papillomavirus (HPV) essential protein which is required for its replication.  RPE1 recognizes the HPV origin of replication (ori) with the aid of '''regulatory protein E2''' (RPE2).  The resultant helicase complex initiates DNA unwinding to provide the template of progeny DNA synthesis,  The HPV ori contains one RPE1 binding site and 2 RPE2 binding sites<ref>PMID:12086149</ref>.  RPE1 is found also in bovine papillomavirus (BPV).
 == Disease ==
-RPE2 induces apoptosis of its host cells<ref>PMID:26906543</ref>.
+RPE1 has been shown to mitigate host cell defenses leading to the development of cancer induces apoptosis of its host cells<ref>PMID:29495005</ref>.
+==Relevance==
+The detection of RPE1 mRNA and promoter methylation may serve as cancer prognostic marker.
 == Structural highlights ==
-The complex between HPV6a DBD and 18-bp DNA (PDB code [[2ayg]]) shows the binding of 2 recognition helices of the dimer to 2 successive major grooves of the target DNA making numerous contacts to the DNA backbone phosphate atoms and to its bases<ref>PMID:16914454</ref>.
+The complex between BPV1 DBD and 21-bp DNA (PDB code [[1ksx]]) shows 2 DBD-DNA binding modules: a binding loop and a binding helix each interacting with a single strand. The protein-DNA interactions are hydrophilic to phosphate atoms and van der Waals hydrophobic ones<ref>PMID:11889054</ref>.
 </StructureSection>
 ==3D structures of replication protein E1==