7b81: Difference between revisions
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==Crystal structure of Azotobacter vinelandii L-rhamnose 1-dehydrogenase (NAD bound-form)== | ==Crystal structure of Azotobacter vinelandii L-rhamnose 1-dehydrogenase (NAD bound-form)== | ||
<StructureSection load='7b81' size='340' side='right'caption='[[7b81]]' scene=''> | <StructureSection load='7b81' size='340' side='right'caption='[[7b81]], [[Resolution|resolution]] 2.09Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B81 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7b81]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Azovd Azovd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7B81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7B81 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b81 OCA], [https://pdbe.org/7b81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b81 RCSB], [https://www.ebi.ac.uk/pdbsum/7b81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b81 ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Avin_09160 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=322710 AZOVD])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7b81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7b81 OCA], [https://pdbe.org/7b81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7b81 RCSB], [https://www.ebi.ac.uk/pdbsum/7b81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7b81 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Several microorganisms can utilize L-rhamnose as a carbon and energy source through the non-phosphorylative metabolic pathway, in which L-rhamnose 1-dehydrogenase (RhaDH) catalyzes the NAD(P)(+) -dependent oxidization of L-rhamnose to L-rhamnono-1,4-lactone. We herein investigated the crystal structures of RhaDH from Azotobacter vinelandii in ligand-free, NAD(+) -bound, NADP(+) -bound, and L-rhamnose- and NAD(+) -bound forms at 1.9, 2.1, 2.4, and 1.6 A resolution, respectively. The significant interactions with the 2'-phosphate group of NADP(+) , but not the 2'-hydroxyl group of NAD(+) , were consistent with a preference for NADP(+) over NAD(+) . The C5-OH and C6-methyl groups of L-rhamnose were recognized by specific residues of RhaDH through hydrogen bonds and hydrophobic contact, respectively, which contribute to the different substrate specificities from other aldose 1-dehydrogenases in the short-chain dehydrogenase/reductase superfamily. | |||
Crystal structure of L-rhamnose 1-dehydrogenase involved in the non-phosphorylative pathway of L-rhamnose metabolism in bacteria.,Yoshiwara K, Watanabe S, Watanabe Y FEBS Lett. 2021 Jan 22. doi: 10.1002/1873-3468.14046. PMID:33482017<ref>PMID:33482017</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7b81" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Azovd]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Watanabe S]] | [[Category: Watanabe, S]] | ||
[[Category: Watanabe Y]] | [[Category: Watanabe, Y]] | ||
[[Category: Yoshiwara K]] | [[Category: Yoshiwara, K]] | ||
[[Category: L-rhanose metabolism]] | |||
[[Category: Nadp-dependent dehydrogenase]] | |||
[[Category: Oxidoreductase]] | |||
[[Category: Sdr protein family]] | |||
Revision as of 06:58, 17 March 2021
Crystal structure of Azotobacter vinelandii L-rhamnose 1-dehydrogenase (NAD bound-form)
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