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| {{STRUCTURE_1dnp| PDB=1dnp | SCENE= }} | | {{STRUCTURE_1dnp| PDB=1dnp | SCENE= }} |
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| '''STRUCTURE OF DEOXYRIBODIPYRIMIDINE PHOTOLYASE'''
| | ===STRUCTURE OF DEOXYRIBODIPYRIMIDINE PHOTOLYASE=== |
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| ==Overview==
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| Photolyase repairs ultraviolet (UV) damage to DNA by splitting the cyclobutane ring of the major UV photoproduct, the cis, syn-cyclobutane pyrimidine dimer (Pyr <> Pyr). The reaction is initiated by blue light and proceeds through long-range energy transfer, single electron transfer, and enzyme catalysis by a radical mechanism. The three-dimensional crystallographic structure of DNA photolyase from Escherichia coli is presented and the atomic model was refined to an R value of 0.172 at 2.3 A resolution. The polypeptide chain of 471 amino acids is folded into an amino-terminal alpha/beta domain resembling dinucleotide binding domains and a carboxyl-terminal helical domain; a loop of 72 residues connects the domains. The light-harvesting cofactor 5,10-methenyltetrahydrofolylpolyglutamate (MTHF) binds in a cleft between the two domains. Energy transfer from MTHF to the catalytic cofactor flavin adenine dinucleotide (FAD) occurs over a distance of 16.8 A. The FAD adopts a U-shaped conformation between two helix clusters in the center of the helical domain and is accessible through a hole in the surface of this domain. Dimensions and polarity of the hole match those of a Pyr <> Pyr dinucleotide, suggesting that the Pyr <> Pyr "flips out" of the helix to fit into this hole, and that electron transfer between the flavin and the Pyr <> Pyr occurs over van der Waals contact distance.
| | The line below this paragraph, {{ABSTRACT_PUBMED_7604260}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 7604260 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_7604260}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Excitation energy transfer]] | | [[Category: Excitation energy transfer]] |
| [[Category: Lyase]] | | [[Category: Lyase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:03:31 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:20:42 2008'' |
