AlphaFold2 examples from CASP 14

This page is under construction. Eric Martz 01:03, 22 February 2021 (UTC)

Prediction of protein structures from amino acid sequences, homology modeling, has been extremely challenging. In 2020, breakthrough success was achieved by AlphaFold2, a project of DeepMind. For an overview of this breakthrough, verified by the bi-annual prediction competition empirical models, please see 7jtl. Below are illustrated some examples of predictions from that competition.

spinqualitylabelsall models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D SARS-CoV-2 ORF8 Following the discussion by Rubiera, our first example will be SARS-CoV-2 protein ORF8, a protein that contributes to virulence in COVID-19. CASP 14 classified ORF8 as a "free modeling" (FM) target, meaning that there were no adequate empirical templates for homology modeling. This was easily confirmed. When the amino acid sequence of ORF8 is submitted to Swiss Model, it reports the best templates for homology modeling. When the two empirical models that were not available during CASP 14 are excluded (7jtl and 7jx6), the best template offered, chain B of 3afc, covers only 36% of the length of ORF8 at 13.2% sequence identity, with a 4-residue untemplated gap in the sequence alignment. This template would not be adequate for constructing a useful model. X-Ray Structures for ORF8 The quality of predictions for the structure of ORF8 are judged by comparison with X-ray crystallographic empirical models which were not available to the groups making predictions. Shortly after the CASP 14 competition (summer 2020), two X-ray crystal structures were reported for ORF8: 7jtl released August 26, 2020, and 7jx6, released September 23, 2020. The resolutions are 2.0 and 1.6 Å respectively, and both have worse than average Rfree values. Click the green links below to change the molecular scene. Drag to rotate. Zoom the molecule with your mouse wheel, or Shift-Drag up/down. The two X-ray structures agree very well. The only substantial disagreement is for an external loop, sequence range 47-58. See the TABLE below for RMSD values. AlphaFold2 Prediction for ORF8 The quality of a prediction in CASP is judged, in large part, by the Global Distance Test Total Score, GDT_TS. AlphaFold2's predicted structure has a GDT_TS score of 87. (A score of 0 is meaningless, and a score of 100 means perfect agreement with an X-ray crystal structure.) 87 means the model is close to the accuracy of an X-ray crystal structure. The structure predicted by AlphaFold2 is almost as close to the X-ray crystallographic model 7jx6 as is the independently-determined X-ray structure 7jtl. AlphaFold2 predicted the positions of 92 amino acids. (CASP 14 excluded residues 48-59, a 12-residue surface loop, from the target residues.) See TABLE below for RMSD values. ORF8 Alignments With Chain A of 7jx6 Model GDT_TS Cα RMSD, Å Cα Aligned RMSD Including Sidechains, Å Atoms Aligned 7jtl:A 4.02 0.66 102/102 (100%) 87/102 (85%) 4.3 1.58 829/829 (100%) 709/829 (86%) AlphaFold2 87 2.58 1.25 92/92 (100%) 83/92* (90%) 3.23 1.91 747/748 (100%) 679/748 (91%) 2nd Best* 43 5.33 1.71 92/92 (100%) 38/92 (41%) 6.54 5.86 747/748 (100%) 324/748 (43%) 3rd Best§ 33 13.37 † 92/92 (100%) † 14.50 † 747/748 (100%) † Alignments by "Magic Fit" of Swiss-PdbViewer 4.1. Alignments by "Iterative Magic Fit" of Swiss-PdbViewer 4.1. *Second best: Group of Xian Ming Pan. §Third best: Group of Perez Lab. † Iterative Magic Fit was unable to align.

Proteopedia Page Contributors and Editors (what is this?)

Eric Martz